NUCB2 ELISA Kit

Catalog No. ABIN4881312

Product Details NUCB2 ELISA Kit

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1-1.000 ng/mL

Minimum Detection Limit: 0.1 ng/mL

Application: ELISA

Purpose: Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.

Sensitivity: 400 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

References for NUCB2 ELISA Kit (ABIN4881312)

Serin, Bakacak, Ercan, Köstü, Avci, Arıkan, Kıran: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).

Abaci, Catli, Anik, Kume, Bober: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." in: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).

Target Details for NUCB2

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Alternative Name: Nesfatin (NES1 Products)

Synonyms: nucleobindin-2 ELISA Kit, nucleobindin 2 ELISA Kit, NUCB2 ELISA Kit, Nucb2 ELISA Kit

Gene ID: 4925

UniProt: P80303