IL-5 ELISA Kit
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- Target See all IL-5 (IL5) ELISA Kits
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 15.6-1000 pg/mL
- Minimum Detection Limit
- 15.6 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of human Interleukin 5,IL-5 concentrations in cell culture supernates, serum, plasma and other biological fluids.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay recognizes recombinant and natural human IL-5.
- Cross-Reactivity (Details)
- No significant cross-reactivity or interference was observed.
- Sensitivity
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
- Characteristics
- Homo sapiens,Human,Interleukin-5,IL-5,B-cell differentiation factor I,Eosinophil differentiation factor,T-cell replacing factor,TRF,IL5
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- Discover our top product IL5 ELISA Kit
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution 2 and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
- Storage Comment
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Target See all IL-5 (IL5) ELISA Kits
- IL-5 (IL5) (Interleukin 5 (IL5))
- Alternative Name
- IL5 (IL5 Products)
- Synonyms
- EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit
- Background
- Il-5 is a 135 amino acid (aa) polypeptide with a predicted mass of 12.5 kDa. It is secreted by a restricted number of mesenchymal cell types. In its native state, mature IL-5 is synthesized as a 115 aa, highly glycosylated 22 kDa monomer that forms a 40-50 kDa disulfide-linked homodimer. Although the content of carbohydrate is high, carbohydrate is not needed for bioactivity. Monomeric IL-5 has no activity and requires a homodimer for function, which is in contrast to the receptor-related IL-3 and GM-CSF cytokines that exist only as monomers. Just as one IL-3 and GM-CSF monomer binds to one receptor, one IL-5 homodimer is able to engage only one IL-5 receptor. It has been suggested that IL-5 (as a dimmer) undergoes a general conformational change after binding to one receptor molecule and this change precludes binding to a second receptor. Mouse and mouse mature IL-5 are 71% identical at the aa level. IL-5 appears to perform a number of functions on eosinophils including growth and differentiation, the down modulation of Mac-1, the upregulation of receptors for IgA and IgG, the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release. IL-5 may act in an adjunct fashion plays, however, the exact role is unclear. Finally, there is a great deal of interest in the interaction between IL-5 and the CC chemokine eotaxin. Studies suggest that inflammatory-induced and locally produced IL-5 and eotaxin may act on the bone marrow in a cooperative manner.
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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