Anti-Beta1 Adrenergic Receptor Antibodies (IgG) ELISA Kit
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- Target
- Anti-Beta1 Adrenergic Receptor Antibodies (IgG)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Purpose
- The anti-beta1-adrenergic-receptor-antibody-EIA is an antibody screening test. The beta1-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-beta1-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-beta1-adrenergic-receptor-antibodies.
- Analytical Method
- Quantitative
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- Plate
- Pre-coated
- Assay Procedure
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Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm - Assay Precision
- Intraassay-Precision:9.58% Interassay-Precision:12.02%
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- Anti-Beta1 Adrenergic Receptor Antibodies (IgG)
- Target Type
- Antibody
- Background
- The beta-1 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The occurrence of auto- antibodies against the beta1-adrenergic-receptor is associated with the existence of ditated cardiomyopathy (DCM). The anti-beta1-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the beta1-adrenergic-receptor in serum and plasma.
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