UCK2 ELISA Kit

Catalog No. ABIN418271

Product Details UCK2 ELISA Kit

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Sensitivity: 0.056 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Application Notes:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Plate: Pre-coated

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 40 ng/mL. Firstly dilute the stock solution to 10 ng/mL and the diluted standard serves as the highest standard (10 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Assay Precision: Intra-assay Precision (precision within an assay): Three samples with low, medium and high levels of the target antigen were tested twenty times on one plate, respectively.
Inter-assay Precision (precision between assays): Three samples with low, medium and high levels of the target antigen were tested on three different plates, eight replicates in each plate.
CV (%) = SD/mean X 100

• Intra-assay: CV less than 10 %
• Inter-assay: CV less than 12 %

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C/-20 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details for UCK2

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Alternative Name: Uridine Cytidine Kinase 2 (UCK2) (UCK2 Products)

Synonyms: TSA903 ELISA Kit, UK ELISA Kit, UMPK ELISA Kit, AA407809 ELISA Kit, AI481316 ELISA Kit, AU018180 ELISA Kit, AU020720 ELISA Kit, UMK ELISA Kit, Umpk ELISA Kit, wu:fa20g04 ELISA Kit, zgc:56174 ELISA Kit, UCK2 ELISA Kit, DDBDRAFT_0202355 ELISA Kit, DDBDRAFT_0216233 ELISA Kit, DDB_0202355 ELISA Kit, DDB_0216233 ELISA Kit, DDBDRAFT_0167798 ELISA Kit, DDBDRAFT_0230208 ELISA Kit, DDB_0167798 ELISA Kit, DDB_0230208 ELISA Kit, UCK 2-A ELISA Kit, umpk ELISA Kit, wu:fk91a01 ELISA Kit, zgc:66240 ELISA Kit, uridine-cytidine kinase 2 ELISA Kit, uridine-cytidine kinase 2b ELISA Kit, microRNA 3658 ELISA Kit, uridine-cytidine kinase 2 S homeolog ELISA Kit, uridine kinase ELISA Kit, Uridine kinase ELISA Kit, toxin secretion ABC transporter ATP-binding protein ELISA Kit, uridine-cytidine kinase 2a ELISA Kit, UCK2 ELISA Kit, Uck2 ELISA Kit, uck2b ELISA Kit, MIR3658 ELISA Kit, uck2.S ELISA Kit, LOC692983 ELISA Kit, PTRG_08949 ELISA Kit, udkA ELISA Kit, udkB ELISA Kit, Mrub_2364 ELISA Kit, Mesil_2200 ELISA Kit, Spirs_2239 ELISA Kit, Tmar_0146 ELISA Kit, Bache_3005 ELISA Kit, Celal_2781 ELISA Kit, Deima_0461 ELISA Kit, Odosp_0439 ELISA Kit, Bacsa_1376 ELISA Kit, Celly_0243 ELISA Kit, Weevi_1813 ELISA Kit, Marky_0973 ELISA Kit, Spico_0967 ELISA Kit, Poras_0295 ELISA Kit, Theth_1915 ELISA Kit, uck2 ELISA Kit, XCC1480 ELISA Kit, uck2a ELISA Kit

Pathways: Ribonucleoside Biosynthetic Process, Feeding Behaviour