CST3 ELISA Kit

Catalog No. ABIN411392

Product Details CST3 ELISA Kit

Target: CST3 (Cystatin C (CST3))

Binding Specificity: AA 1-146

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cystatin C

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: M1-A146

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: M1-A146

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Plate: Pre-coated

Protocol: human Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin C has been precoated onto 96-well plates. Standards(NSO, M1-A146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cystatin C amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Cystatin C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cystatin C standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 2.68, Standard deviation: 0.115, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 6.13, Standard deviation: 0.288, CV(%): 4.7
• Sample 3: n=16, Mean(ng/ml): 12.83, Standard deviation: 0.744, CV(%): 5.8,
• Sample 1: n=24, Mean(ng/ml): 2.87, Standard deviation: 0.152, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 6.24, Standard deviation: 0.356, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 13.21, Standard deviation: 0.845, CV(%): 6.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for CST3 ELISA Kit (ABIN411392)

Chen, Lu, Wang, Bian, Cheng, Su, Zhou: "Elevated urinary levels of cystatin C and neutrophil gelatinase-associated lipocalin in Henoch-Schönlein purpura patients with renal involvement." in: PLoS ONE, Vol. 9, Issue 6, pp. e101026, (2014) (PubMed).

Target Details for CST3

Target: CST3 (Cystatin C (CST3))

Alternative Name: CST3 (CST3 Products)

Synonyms: armd11 ELISA Kit, fb51d07 ELISA Kit, wu:fb24g06 ELISA Kit, wu:fb51d07 ELISA Kit, wu:fc55f03 ELISA Kit, zgc:136227 ELISA Kit, ARMD11 ELISA Kit, CysC ELISA Kit, CYSC ELISA Kit, cystatin C ELISA Kit, cystatin C L homeolog ELISA Kit, cystatin C (amyloid angiopathy and cerebral hemorrhage) ELISA Kit, cystatin 3 ELISA Kit, CST3 ELISA Kit, cst3.L ELISA Kit, cst3 ELISA Kit, cystatin 3 ELISA Kit, Cst3 ELISA Kit

Background:

Protein Function: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Background: Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Synonyms: Cystatin-C,Cystatin-3,Gamma-trace,Neuroendocrine basic polypeptide,Post-gamma-globulin,CST3,

Full Gene Name: Cystatin-C

Cellular Localisation: Secreted.

Gene ID: 1471

UniProt: P01034