LTA ELISA Kit

Catalog No. ABIN411381

Product Details LTA ELISA Kit

Target: LTA (Lymphotoxin-alpha (LTA))

Binding Specificity: AA 35-205

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF beta

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <5pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the tumor necrosis factor family.

Plate: Pre-coated

Protocol: human TNF beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF beta has been precoated onto 96-well plates. Standards(E.coli, L35-L205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNF beta amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 415, Standard deviation: 23.24, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 1952, Standard deviation: 68.32, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 2822, Standard deviation: 129.8, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 660, Standard deviation: 40.92, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 2546, Standard deviation: 122.2, CV(%): 4.8
• Sample 3: n=24, Mean(pg/ml): 3148, Standard deviation: 210.9, CV(%): 6.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for LTA ELISA Kit (ABIN411381)

Wang, Zheng, Meng, Wang, He, Zhang, Liu, Hu, He, Hu, Wang: "In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus." in: Biomaterials, Vol. 64, pp. 78-87, (2015) (PubMed).

Zeng, Huang, Zheng, Guo, Pan: "Effects of reactive nitrogen scavengers on NK-cell-mediated killing of K562 cells." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 101737, (2012) (PubMed).

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: LTA (LTA Products)

Synonyms: LT ELISA Kit, TNFB ELISA Kit, TNFSF1 ELISA Kit, LT-[a] ELISA Kit, LT-alpha ELISA Kit, LT[a] ELISA Kit, LTalpha ELISA Kit, Ltx ELISA Kit, TNF-beta ELISA Kit, Tnfb ELISA Kit, Tnfsf1b ELISA Kit, hlb382 ELISA Kit, lymphotoxin alpha ELISA Kit, lymphotoxin A ELISA Kit, LTA ELISA Kit, Lta ELISA Kit

Target Type: Chemical

Background:

Protein Function: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Synonyms: Lymphotoxin-alpha,LT-alpha,TNF-beta,Tumor necrosis factor ligand superfamily member 1,LTA,TNFB, TNFSF1,

Full Gene Name: Lymphotoxin-alpha

Cellular Localisation: Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.

Gene ID: 4049

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process