Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TIMP3 ELISA Kit

TIMP3 Reactivity: Human AA 24-211 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Catalog No. ABIN411360
  • Target See all TIMP3 ELISA Kits
    TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))
    Binding Specificity
    AA 24-211
    Reactivity
    • 10
    • 7
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: C24-P211
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <2pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: C24-P211
    Featured
    Discover our best selling TIMP3 ELISA Kit
    Top Product
    Discover our top product TIMP3 ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

    Plate
    Pre-coated
    Protocol
    human TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NSO, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1250, Standard deviation: 72.5, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 2687, Standard deviation: 110.2, CV(%): 4.1
    • Sample 3: n=16, Mean(pg/ml): 5353, Standard deviation: 192.7, CV(%): 3.6,
    • Sample 1: n=24, Mean(pg/ml): 1430, Standard deviation: 95.81, CV(%): 6.7
    • Sample 2: n=24, Mean(pg/ml): 2832, Standard deviation: 147.3, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 5657, Standard deviation: 277.2, CV(%): 4.9
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Denkovskij, Bagdonas, Kusleviciute, Mackiewicz, Unguryte, Porvaneckas, Fleury, Venalis, Jorgensen, Bernotiene: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).

  • Target See all TIMP3 ELISA Kits
    TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))
    Alternative Name
    TIMP3 (TIMP3 Products)
    Synonyms
    TIMP-3 ELISA Kit, TIMP3 ELISA Kit, timp3 ELISA Kit, HSMRK222 ELISA Kit, K222 ELISA Kit, K222TA2 ELISA Kit, SFD ELISA Kit, IMP-3 ELISA Kit, timp3-A ELISA Kit, Timp-3 ELISA Kit, metalloproteinase inhibitor 3 ELISA Kit, TIMP metallopeptidase inhibitor 3 ELISA Kit, Metalloproteinase inhibitor 3 ELISA Kit, TIMP metallopeptidase inhibitor 3 L homeolog ELISA Kit, tissue inhibitor of metalloproteinase 3 ELISA Kit, CpipJ_CPIJ003282 ELISA Kit, TIMP3 ELISA Kit, timp3 ELISA Kit, Timp3 ELISA Kit, timp3.L ELISA Kit
    Background

    Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

    Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

    Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,

    Full Gene Name: Metalloproteinase inhibitor 3

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    Gene ID
    7078
    UniProt
    P35625
You are here:
Support