TIMP3 ELISA Kit

Catalog No. ABIN411360

Product Details TIMP3 ELISA Kit

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Binding Specificity: AA 24-211

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-3

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: C24-P211

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <2pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: C24-P211

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Plate: Pre-coated

Protocol: human TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NSO, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1250, Standard deviation: 72.5, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 2687, Standard deviation: 110.2, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 5353, Standard deviation: 192.7, CV(%): 3.6,
• Sample 1: n=24, Mean(pg/ml): 1430, Standard deviation: 95.81, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 2832, Standard deviation: 147.3, CV(%): 5.2
• Sample 3: n=24, Mean(pg/ml): 5657, Standard deviation: 277.2, CV(%): 4.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TIMP3 ELISA Kit (ABIN411360)

Denkovskij, Bagdonas, Kusleviciute, Mackiewicz, Unguryte, Porvaneckas, Fleury, Venalis, Jorgensen, Bernotiene: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3 (TIMP3 Products)

Synonyms: TIMP-3 ELISA Kit, TIMP3 ELISA Kit, timp3 ELISA Kit, HSMRK222 ELISA Kit, K222 ELISA Kit, K222TA2 ELISA Kit, SFD ELISA Kit, IMP-3 ELISA Kit, timp3-A ELISA Kit, Timp-3 ELISA Kit, metalloproteinase inhibitor 3 ELISA Kit, TIMP metallopeptidase inhibitor 3 ELISA Kit, Metalloproteinase inhibitor 3 ELISA Kit, TIMP metallopeptidase inhibitor 3 L homeolog ELISA Kit, tissue inhibitor of metalloproteinase 3 ELISA Kit, CpipJ_CPIJ003282 ELISA Kit, TIMP3 ELISA Kit, timp3 ELISA Kit, Timp3 ELISA Kit, timp3.L ELISA Kit

Background:

Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,

Full Gene Name: Metalloproteinase inhibitor 3

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 7078

UniProt: P35625