Interleukin 17a ELISA Kit

Catalog No. ABIN411314

Product Details Interleukin 17a ELISA Kit

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Binding Specificity: AA 20-155

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: I20-A155

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: I20-A155

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-17 family.

Tissue Specificity: Restricted to activated memory T-cells.

Plate: Pre-coated

Protocol: human IL-17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17 has been precoated onto 96-well plates. Standards(E.coli, I20-A155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-17 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 147, Standard deviation: 6.91, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 782, Standard deviation: 39.1, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 1434, Standard deviation: 64.53, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 158, Standard deviation: 12.5, CV(%): 7.9
• Sample 2: n=24, Mean(pg/ml): 825, Standard deviation: 70.95, CV(%): 8.6
• Sample 3: n=24, Mean(pg/ml): 1323, Standard deviation: 95.3, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Interleukin 17a ELISA Kit (ABIN411314)

Badal, Kumar, Paul, Dayal, Bhansali, Bhadada, Kumar, Sachdeva et al.: "Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in ..." in: The Indian journal of medical research, Vol. 145, Issue 6, pp. 767-776, (2018) (PubMed).

Min, Yan, Wang, Landeck, Chen, Li, Cai, Zheng, Man: "Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies." in: PLoS ONE, Vol. 12, Issue 12, pp. e0190051, (2018) (PubMed).

Yuksel, Kaplan, Ates, Kilic, Kilic, Suna, Ates, Kayacetin: "The role of soluble tumor necrosis factor like weak inducer of apoptosis and interleukin-17A in the etiopathogenesis of celiac disease: A cross-sectional study." in: Medicine, Vol. 95, Issue 26, pp. e3937, (2017) (PubMed).

Choghakhori, Abbasnezhad, Hasanvand, Amani: "Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life." in: Cytokine, Vol. 93, pp. 34-43, (2017) (PubMed).

Romano, Sollazzo, Trabanelli, Barone, Polverelli, Perricone, Forte, Luatti, Cavo, Vianelli, Jandus, Palandri, Catani: "Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis." in: Oncoimmunology, Vol. 6, Issue 10, pp. e1345402, (2017) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "Changes in interleukin-17 and transforming growth factor beta 1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma." in: Translational pediatrics, Vol. 2, Issue 4, pp. 154-9, (2016) (PubMed).

Cam, Karagoz, Muftuoglu, Bigi, Emirzeoglu, Celik, Ozgun, Tuncel, Top: "The inflammatory cytokine interleukin-23 is elevated in lung cancer, particularly small cell type." in: Contemporary oncology (Poznań, Poland), Vol. 20, Issue 3, pp. 215-9, (2016) (PubMed).

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Lu, Zeng, Han, Lin, Long, Dan, Zhou, Chen: "Overexpression and selectively regulatory roles of IL-23/IL-17 axis in the lesions of oral lichen planus." in: Mediators of inflammation, Vol. 2014, pp. 701094, (2014) (PubMed).

Chai, Yang, Gao, Guo, Li, Fan, Wu, Xue, Cai, Jiang, Qin, Zhang, Ke: "Decreased percentages of regulatory T cells are necessary to activate Th1-Th17-Th22 responses during acute rejection of the peripheral nerve xenotransplantation in mice." in: Transplantation, Vol. 98, Issue 7, pp. 729-37, (2014) (PubMed).

Jin, Wan, Chen, Chen, Zhang, Deng, Zhang, Xiong, Xin: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Sereshki, Gharagozloo, Ostadi, Ghahiri, Roghaei, Mehrabian, Andalib, Hassanzadeh, Hosseini, Rezaei: "Variations in T-helper 17 and Regulatory T Cells during The Menstrual Cycle in Peripheral Blood of Women with Recurrent Spontaneous Abortion." in: International journal of fertility & sterility, Vol. 8, Issue 1, pp. 59-66, (2014) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "[Changes in IL-17 and TGF-?1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma]." in: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, Vol. 15, Issue 8, pp. 604-8, (2013) (PubMed).

Xu, Tao, Zhang, Zhang: "Neutralization of interleukin-17 attenuates high fat diet-induced non-alcoholic fatty liver disease in mice." in: Acta biochimica et biophysica Sinica, Vol. 45, Issue 9, pp. 726-33, (2013) (PubMed).

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, (2013) (PubMed).

Liu, Ge, Ma, Mei, Liu, Zhang, Ren, Liao, Pu, Wang, You: "Interleukin-17 and prostaglandin E2 are involved in formation of an M2 macrophage-dominant microenvironment in lung cancer." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 7, Issue 7, pp. 1091-100, (2012) (PubMed).

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A (IL17A Products)

Synonyms: CTLA8 ELISA Kit, IL-17 ELISA Kit, IL-17A ELISA Kit, IL17 ELISA Kit, Ctla-8 ELISA Kit, Ctla8 ELISA Kit, Il17 ELISA Kit, ChIL-17 ELISA Kit, IL-17F ELISA Kit, IL17A ELISA Kit, CTLA-8 ELISA Kit, interleukin 17A ELISA Kit, IL17A ELISA Kit, Il17a ELISA Kit

Background:

Protein Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

Background: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57 % identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Synonyms: Interleukin-17A,IL-17,IL-17A,Cytotoxic T-lymphocyte-associated antigen 8,CTLA-8,IL17A,CTLA8, IL17,

Full Gene Name: Interleukin-17A

Cellular Localisation: Secreted.

Gene ID: 3605

UniProt: Q16552