IL-10 ELISA Kit

Catalog No. ABIN411308

Product Details IL-10 ELISA Kit

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Binding Specificity: AA 19-178

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-10

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <4pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-10 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-10 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 277, Standard deviation: 13.85, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 821, Standard deviation: 34.5, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 1344, Standard deviation: 52.42, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 316, Standard deviation: 29.1, CV(%): 9.2
• Sample 2: n=24, Mean(pg/ml): 938, Standard deviation: 70.35, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 1620, Standard deviation: 144.2, CV(%): 8.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-10 ELISA Kit (ABIN411308)

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Gao, Meng, Cui: "Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat." in: Experimental lung research, Vol. 43, Issue 9-10, pp. 439-446, (2018) (PubMed).

He, Cui, Wang, Yan, Li, Gong, Zheng, Luo, Wang: "Modulation of inflammation by toll-like receptor 4/nuclear factor-kappa B in diarrhea-predominant irritable bowel syndrome." in: Oncotarget, Vol. 8, Issue 69, pp. 113957-113965, (2018) (PubMed).

Gao, Jing, Zhang, Wu et al.: "Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor ..." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1029-1035, (2018) (PubMed).

Zhu, Guo, Fang, Cao, Yan, Chen, Zhang, Zhang: "Kupffer cell depletion by gadolinium chloride aggravates liver injury after brain death in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6357-6362, (2018) (PubMed).

Sarandy, Miranda, Altoé, Novaes, Zanuncio, Leite, Gonçalves: "Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195786, (2018) (PubMed).

Lin, Ju, Gao, Li, Guo: "Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome." in: BioMed research international, Vol. 2018, pp. 7507314, (2018) (PubMed).

Chen, Chen, Fan, Wu, Yang, Fang, Fu, Li et al.: "Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental ..." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 116, (2018) (PubMed).

Liu, Xu, Yin, Wang, Wang, Xiao: "IL-10/STAT3 is reduced in childhood obesity with hypertriglyceridemia and is related to triglyceride level in diet-induced obese rats." in: BMC endocrine disorders, Vol. 18, Issue 1, pp. 39, (2018) (PubMed).

Ye, Shen, Huang, Zhang, Xiao: "Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model." in: Journal of the science of food and agriculture, Vol. 97, Issue 13, pp. 4548-4556, (2017) (PubMed).

Mansour, El-Marakby, Moawed: "Ameliorative effects of rutin on hepatic encephalopathy-induced by thioacetamide or gamma irradiation." in: Journal of photochemistry and photobiology. B, Biology, Vol. 172, pp. 20-27, (2017) (PubMed).

Kang, Lu, Zhan, Liang, Guo, Ma, Wang, Song, Feng, Wang, Bai, Song, Liu: "Dai-Huang-Fu-Zi-Tang alleviates pulmonary and intestinal injury with severe acute pancreatitis via regulating aquaporins in rats." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 288, (2017) (PubMed).

Korelo, Kryczyk, Garcia, Naliwaiko, Fernandes: "Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats." in: Brazilian journal of physical therapy, Vol. 20, Issue 2, pp. 133-41, (2017) (PubMed).

Xu, Huang, Zhang, Li, Zheng, Yu, Chu, Lin, Chen: "Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4167-74, (2017) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Mirmalek, Gholamrezaei Boushehrinejad, Yavari, Kardeh, Parsa, Salimi-Tabatabaee, Yadollah-Damavandi, Parsa, Shahverdi, Jangholi: "Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5818479, (2017) (PubMed).

Zhao, Pan, Wang, Xu, Song: "Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model." in: Yonsei medical journal, Vol. 58, Issue 1, pp. 131-138, (2017) (PubMed).

Zhang, Li, Li, Liu, Guo, Xu: "Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models." in: Frontiers in physiology, Vol. 8, pp. 249, (2017) (PubMed).

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Chen, Chen, Li, Dong, Teng, Nong, Pan, Lv, Gao, Wu: "Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy." in: Oncotarget, Vol. 8, Issue 67, pp. 111522-111534, (2017) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10 (IL10 Products)

Synonyms: CSIF ELISA Kit, GVHDS ELISA Kit, IL-10 ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Il-10 ELISA Kit, IL10X ELISA Kit, interleukin 10 ELISA Kit, IL10 ELISA Kit, Il10 ELISA Kit

Background:

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .

Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10,

Full Gene Name: Interleukin-10

Cellular Localisation: Secreted.

Gene ID: 25325

UniProt: P29456

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints