IL-10 ELISA Kit

Catalog No. ABIN411306

Product Details IL-10 ELISA Kit

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Binding Specificity: AA 19-178

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-10

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <0.5pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-10 family.

Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Plate: Pre-coated

Protocol: human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-10 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-10 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 25, Standard deviation: 1.35, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 112, Standard deviation: 5.38, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 280, Standard deviation: 8.68, CV(%): 3.1,
• Sample 1: n=24, Mean(pg/ml): 34, Standard deviation: 2.55, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 184, Standard deviation: 14.54, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 312, Standard deviation: 20.9, CV(%): 6.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-10 ELISA Kit (ABIN411306)

You, Tan, Dai, Chen, Xu, Yang, Zhang, Bai, Bie: "MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma." in: Oncotarget, Vol. 7, Issue 35, pp. 57099-57116, (2018) (PubMed).

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Badal, Kumar, Paul, Dayal, Bhansali, Bhadada, Kumar, Sachdeva et al.: "Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in ..." in: The Indian journal of medical research, Vol. 145, Issue 6, pp. 767-776, (2018) (PubMed).

Dang, Xie, Zhou: "Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes." in: Chinese journal of integrative medicine, Vol. 24, Issue 1, pp. 24-31, (2018) (PubMed).

Ma, Zhang, Zhao, Wang, Lin, Ma: "Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 4671-4676, (2018) (PubMed).

Choghakhori, Abbasnezhad, Hasanvand, Amani: "Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life." in: Cytokine, Vol. 93, pp. 34-43, (2017) (PubMed).

Gheybi, Farrokhi, Ravanbod, Ostovar, Mehrzad, Nematollahi: "The correlation of CD19 + CD24 + CD38 + B cells and other clinicopathological variables with the proportion of circulating Tregs in breast cancer patients." in: Breast cancer (Tokyo, Japan), (2017) (PubMed).

Wang, Xie, Yang, Zhang, Wang, Wu, Shen, Xie: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, (2017) (PubMed).

Li, Tang, Shuai, Jiang, Liu, Wang, Yao, Nie, Xie: "Mannose Receptor Mediates the Immune Response to Ganoderma atrum Polysaccharides in Macrophages." in: Journal of agricultural and food chemistry, Vol. 65, Issue 2, pp. 348-357, (2016) (PubMed).

Yao, Li, Wu, Zhang, Wang: "Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4545-54, (2016) (PubMed).

Zhang, Li, Li, Shi, Zhou, Fu, Zhang, Yang, Fu, Lu: "Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis." in: Scientific reports, Vol. 5, pp. 18398, (2016) (PubMed).

Zang, Jiang, Li, DU, Niu: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).

Chen, Guo, Yang, Zheng, Zeng, Tong: "Protective effect of ginsenoside Rb1 on integrity of blood-brain barrier following cerebral ischemia." in: Experimental brain research, Vol. 233, Issue 10, pp. 2823-31, (2015) (PubMed).

Meng, Wang, Zhang, Qi, Liu, Wu: "Additive Suppression of LPS-Induced IL-10 and TNF-? by Pre-treatment of Dexamethasone and SB203580 in a Murine Alveolar Macrophage Cell Line (MH-S)." in: Inflammation, Vol. 38, Issue 3, pp. 1260-6, (2015) (PubMed).

Luo, Wang, Wang, Chen, Wang, Xu, Chen, Zeng: "Chronic NF-?B blockade improves renal angiotensin II type 1 receptor functions and reduces blood pressure in Zucker diabetic rats." in: Cardiovascular diabetology, Vol. 14, pp. 76, (2015) (PubMed).

Tang, Gao, Wang, Yuan, Ye, Chong, Xu, Jiang, Li, Yin, Miao, Wang, Jiang: "Apoptosis and anergy of T cell induced by pancreatic stellate cells-derived galectin-1 in pancreatic cancer." in: Tumour biology, Vol. 36, Issue 7, pp. 5617-26, (2015) (PubMed).

Berni Canani, Paparo, Nocerino, Cosenza, Pezzella, Di Costanzo, Capasso, Del Monaco, DArgenio, Greco, Salvatore: "Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 38, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Zhang, Zhang, Li, Huang, Xu, Chu, Chen, Chen: "Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injury." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 988-94, (2015) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10 (IL10 Products)

Synonyms: CSIF ELISA Kit, GVHDS ELISA Kit, IL-10 ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Il-10 ELISA Kit, IL10X ELISA Kit, interleukin 10 ELISA Kit, IL10 ELISA Kit, Il10 ELISA Kit

Background:

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,IL10,

Full Gene Name: Interleukin-10

Cellular Localisation: Secreted.

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints