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IL-3 ELISA Kit

IL-3 Reactivity: Mouse AA 27-166 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
Catalog No. ABIN411298
  • Target See all IL-3 ELISA Kits
    IL-3 (Interleukin 3 (IL-3))
    Binding Specificity
    AA 27-166
    Reactivity
    • 13
    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8-500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli,A27-C166
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <1pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: A27-C166
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Activated T-cells, mast cells, natural killer cells.

    Plate
    Pre-coated
    Protocol
    mouse IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-3 has been precoated onto 96-well plates. Standards (E.coli,A27-C166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse IL-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-3 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 32.6, Standard deviation: 1.793, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 156, Standard deviation: 9.516, CV(%): 6.1
    • Sample 3: n=16, Mean(pg/ml): 364, Standard deviation: 23.66, CV(%): 6.5,
    • Sample 1: n=24, Mean(pg/ml): 43.7, Standard deviation: 2.75, CV(%): 6.3
    • Sample 2: n=24, Mean(pg/ml): 164, Standard deviation: 11.81, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 348, Standard deviation: 26.1, CV(%): 7.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

    Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

  • Target See all IL-3 ELISA Kits
    IL-3 (Interleukin 3 (IL-3))
    Alternative Name
    IL3 (IL-3 Products)
    Synonyms
    BPA ELISA Kit, Csfmu ELISA Kit, HCGF ELISA Kit, Il-3 ELISA Kit, MCGF ELISA Kit, PSF ELISA Kit, IL-3 ELISA Kit, MULTI-CSF ELISA Kit, interleukin 3 ELISA Kit, interleukin 3 (colony-stimulating factor, multiple) ELISA Kit, Il3 ELISA Kit, IL3 ELISA Kit
    Background

    Protein Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

    Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.

    Synonyms: Interleukin-3,IL-3,Hematopoietic growth factor,Mast cell growth factor,MCGF,Multipotential colony-stimulating factor,P-cell-stimulating factor,Il3,Csfmu, Il-3,

    Full Gene Name: Interleukin-3

    Cellular Localisation: Secreted.
    Gene ID
    16187
    UniProt
    P01586
    Pathways
    JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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