IL-2 ELISA Kit

Catalog No. ABIN411295

Product Details IL-2 ELISA Kit

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Binding Specificity: AA 21-153

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli,A21-T153

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A21-T153

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-2 family.

Plate: Pre-coated

Protocol: human IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-T153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 75, Standard deviation: 3.45, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 272, Standard deviation: 9.52, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 537, Standard deviation: 16.65, CV(%): 3.1,
• Sample 1: n=24, Mean(pg/ml): 96, Standard deviation: 6.048, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 310, Standard deviation: 16.12, CV(%): 5.2
• Sample 3: n=24, Mean(pg/ml): 643, Standard deviation: 39.2, CV(%): 6.1

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-2 ELISA Kit (ABIN411295)

Han, Wen, Li, Fan, Zhang, Li, Sun, Geng, Sun, Huang, Wu, Lu, Zheng: "Effect of different anesthetic methods on cellular immune functioning and the prognosis of patients with ovarian cancer undergoing oophorectomy." in: Bioscience reports, Vol. 37, Issue 5, (2018) (PubMed).

Zang, Jiang, Li, DU, Niu: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).

Tong, Zhang, Zhao, Liu, Han: "Traffic-related PM2.5 induces cytosolic [Ca²?] increase regulated by Orai1, alters the CaN-NFAT signaling pathway, and affects IL-2 and TNF-? cytoplasmic levels in Jurkat T-cells." in: Archives of environmental contamination and toxicology, Vol. 68, Issue 1, pp. 31-7, (2015) (PubMed).

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." in: BMC complementary and alternative medicine, Vol. 15, pp. 119, (2015) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Eker, Yavasci, Cangur, Kirli, Sarandol: "Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?" in: Acta neuropsychiatrica, Vol. 26, Issue 5, pp. 291-7, (2014) (PubMed).

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Li, He, Wang, Shu, Liu: "USP2a positively regulates TCR-induced NF-?B activation by bridging MALT1-TRAF6." in: Protein & cell, Vol. 4, Issue 1, pp. 62-70, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Zhong, Cheng, Long, He, Qi, Xiang, Zhao, Zhu: "Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma." in: Journal of translational medicine, Vol. 11, pp. 71, (2013) (PubMed).

Sofra, Fei, Fabrizi, Marcelli, Claroni, Gallucci, Ensoli, Forastiere: "Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical cystectomy: preliminary results." in: Journal of experimental & clinical cancer research : CR, Vol. 32, pp. 6, (2013) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Liu, Shen, Wei, Mao, Huang: "Immunomodulatory activity of butanol extract from Solanum lyratum in tumor-bearing mice." in: Immunopharmacology and immunotoxicology, Vol. 33, Issue 1, pp. 100-6, (2011) (PubMed).

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Chen, Ni, Ye, Sun, Li, Xu: "Comparison of immunosuppressive activity of stephanoside E and its aglycone from Stephanotis mucronata in vitro." in: International immunopharmacology, Vol. 10, Issue 10, pp. 1153-60, (2010) (PubMed).

Li, Liang, Ma, Hu, Yan, Li, Fang, Wang, Zhang, Xie, Zhang: "Calcineurin mediates the immune response of hemocytes through NF-kappaB signaling pathway in pearl oyster (Pinctada fucata)." in: Fish & shellfish immunology, Vol. 28, Issue 2, pp. 253-60, (2010) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2 (IL2 Products)

Synonyms: IL2 ELISA Kit, IL-2 ELISA Kit, il2 ELISA Kit, TCGF ELISA Kit, lymphokine ELISA Kit, Il-2 ELISA Kit, POIL2 ELISA Kit, interleukin 2 ELISA Kit, interleukin-15 ELISA Kit, IL2 ELISA Kit, il2 ELISA Kit, LOC100303688 ELISA Kit, Il2 ELISA Kit

Background:

Protein Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Background: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Synonyms: Interleukin-2,IL-2,T-cell growth factor,TCGF,Aldesleukin,IL2,

Full Gene Name: Interleukin-2

Cellular Localisation: Secreted.

Gene ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation