IL-1 beta ELISA Kit

Catalog No. ABIN411294

Product Details IL-1 beta ELISA Kit

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Binding Specificity: AA 118-269

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5-800 pg/mL

Minimum Detection Limit: 12.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 beta

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli,V118-S269

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V118-S269

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V118-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 beta amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 800pg/mL, 400pg/mL, 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-1 betastandard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 86, Standard deviation: 6.97, CV(%): 8.1
• Sample 2: n=16, Mean(pg/ml): 376, Standard deviation: 19.55, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 587, Standard deviation: 24.07, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 79, Standard deviation: 7.03, CV(%): 8.9
• Sample 2: n=24, Mean(pg/ml): 346, Standard deviation: 25.6, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 558, Standard deviation: 37.94, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-1 beta ELISA Kit (ABIN411294)

Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." in: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." in: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Liu, Zhang, Wu, Gong: "Foxo3a-dependent Bim transcription protects mice from a high fat diet via inhibition of activation of the NLRP3 inflammasome by facilitating autophagy flux in Kupffer cells." in: Oncotarget, Vol. 8, Issue 21, pp. 34258-34267, (2018) (PubMed).

Hu, Shen, Wang, Zhao: "Liver X Receptor Agonist TO901317 Attenuates Paraquat-Induced Acute Lung Injury through Inhibition of NF-κB and JNK/p38 MAPK Signal Pathways." in: BioMed research international, Vol. 2017, pp. 4652695, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Feng, Wei, Wang, Fu, Shi, Qu, Fan: "Rhein ameliorates adenomyosis by inhibiting NF-κB and β-Catenin signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 94, pp. 231-237, (2018) (PubMed).

Wang, Dong, Wang, Liu, Sun, Li, Lin, Geng, Jin, Li: "Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult male mice." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 62, pp. 37-45, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." in: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wu, Zhang, Qi, Zhang, Li, Li, Lin, Bai, Liu, Chen, Yang, Xu, Zhang, Yang: "Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis." in: Cell death & disease, Vol. 9, Issue 2, pp. 171, (2018) (PubMed).

Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Zhang, Feng, Cui, Yang, Zhu et al.: "Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box ..." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5805-5813, (2018) (PubMed).

Lu, Zhang, Zhang, Zhou, Gao, Liu, Wang, Wu, Li, Hang: "Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 87, (2018) (PubMed).

Xu, Liang, Li, Ma: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).

Xianchu, Ming, Xiangbin, Lan: "Grape seed proanthocyanidin extract supplementation affects exhaustive exercise-induced fatigue in mice." in: Food & nutrition research, Vol. 62, (2018) (PubMed).

Song, Pei, Hu, Pan, Xiong, Liu, Wu, Shang, Yao: "Death-associated protein kinase 1 mediates interleukin-1β production through regulating inlfammasome activation in Bv2 microglial cells and mice." in: Scientific reports, Vol. 8, Issue 1, pp. 9930, (2018) (PubMed).

Zhang, Li, Liu, Chen, Gong: "Activation of imidazoline I1 receptor by moxonidine regulates the progression of liver fibrosis in the Nrf2-dependent pathway." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 821-834, (2017) (PubMed).

Yang, Dong, Hu, Chen, Fu, Wang, Xu, Tu: "Liver Kinase B1/AMP-Activated Protein Kinase Pathway Activation Attenuated the Progression of Endotoxemia in the Diabetic Mice." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 42, Issue 2, pp. 761-779, (2017) (PubMed).

Jin, Liu, Chen, Xiang, Ding, Li: "Effect of miR-146 targeted HDMCP up-regulation in the pathogenesis of nonalcoholic steatohepatitis." in: PLoS ONE, Vol. 12, Issue 3, pp. e0174218, (2017) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B (IL1B Products)

Synonyms: IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit

Background:

Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Synonyms: Interleukin-1 beta,IL-1 beta,Il1b,

Full Gene Name: Interleukin-1 beta

Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Gene ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome