GDNF ELISA Kit

Catalog No. ABIN411276

Product Details GDNF ELISA Kit

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Binding Specificity: AA 78-211

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat GDNF

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21
Immunogen sequence: S78-I211

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <4pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: S78-I211

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards(sf21, S78-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GDNF amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat GDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat GDNF standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 155, Standard deviation: 6.98, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 624, Standard deviation: 26.21, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 1316, Standard deviation: 72.38, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 148, Standard deviation: 10.06, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 747, Standard deviation: 59.01, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 1324, Standard deviation: 102, CV(%): 7.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for GDNF ELISA Kit (ABIN411276)

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Zhuang, Bu, Hua, Darabi, Cao, Xing: "Gelatin-methacrylamide gel loaded with microspheres to deliver GDNF in bilayer collagen conduit promoting sciatic nerve growth." in: International journal of nanomedicine, Vol. 11, pp. 1383-94, (2016) (PubMed).

Ziv-Polat, Shahar, Levy, Skaat, Neuman, Fregnan, Geuna, Grothe, Haastert-Talini, Margel: "The role of neurotrophic factors conjugated to iron oxide nanoparticles in peripheral nerve regeneration: in vitro studies." in: BioMed research international, Vol. 2014, pp. 267808, (2015) (PubMed).

Morano, Wrobel, Fregnan, Ziv-Polat, Shahar, Ratzka, Grothe, Geuna, Haastert-Talini: "Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials." in: International journal of nanomedicine, Vol. 9, pp. 5289-306, (2015) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." in: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." in: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." in: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." in: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF (GDNF Products)

Synonyms: gdnf ELISA Kit, GDNF ELISA Kit, AI385739 ELISA Kit, gdnfa ELISA Kit, hfb1-gdnf ELISA Kit, ATF1 ELISA Kit, ATF2 ELISA Kit, HFB1-GDNF ELISA Kit, HSCR3 ELISA Kit, glial cell derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor a ELISA Kit, glial cell line derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor L homeolog ELISA Kit, Gdnf ELISA Kit, gdnfa ELISA Kit, GDNF ELISA Kit, gdnf.L ELISA Kit

Background:

Protein Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Background: Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

Synonyms: Glial cell line-derived neurotrophic factor ,Gdnf ,

Full Gene Name: Glial cell line-derived neurotrophic factor

Cellular Localisation: Secreted.

Gene ID: 25453

UniProt: A7UGJ1

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration