Fibronectin 1 ELISA Kit

Catalog No. ABIN411271

Product Details Fibronectin 1 ELISA Kit

Target: Fibronectin 1 (FN1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fibronectin

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: from plasma

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: from plasma

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 12 fibronectin type-I domains.

Tissue Specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. .

Plate: Pre-coated

Protocol: human Fibronectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from rabbit specific for Fibronectin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibronectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Fibronectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Fibronectin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.063, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 2.35, Standard deviation: 0.099, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 5.53, Standard deviation: 0.265, CV(%): 4.8,
• Sample 1: n=24, Mean(ng/ml): 1.54, Standard deviation: 0.120, CV(%): 7.8
• Sample 2: n=24, Mean(ng/ml): 2.39, Standard deviation: 0.177, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 5.78, Standard deviation: 0.399, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Fibronectin 1 ELISA Kit (ABIN411271)

Stevens, Scull, Ramanan, Fortin, Chaturvedi, Knouse, Xiao, Fung, Mirabella, Chen, McCue, Yang, Fleming, Chung, de Jong, Chen, Rice, Bhatia: "In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease." in: Science translational medicine, Vol. 9, Issue 399, (2018) (PubMed).

Banville, Burgess, Jaffar, Tjin, Richeldi, Cerri, Persiani, Black, Oliver: "A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM)." in: PLoS ONE, Vol. 9, Issue 8, pp. e105365, (2014) (PubMed).

Lv, Wu, Zhou, Shao, Wang, Wang: "Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway." in: International journal of endocrinology, Vol. 2014, pp. 658589, (2014) (PubMed).

Xu, Guan, Zheng, Gao, Zeng, Qin, Xue: "Exendin-4 alleviates high glucose-induced rat mesangial cell dysfunction through the AMPK pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 2, pp. 423-32, (2014) (PubMed).

Inoue, Sasaki, Katada, Taguchi: "Quantitative biocompatibility evaluation of nickel-free high-nitrogen stainless steel in vitro/in vivo." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 1, pp. 68-72, (2014) (PubMed).

Zirkel, Lederer, Stöhr, Pazaitis, Hüttelmaier: "IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG)." in: Nucleic acids research, Vol. 41, Issue 13, pp. 6618-36, (2013) (PubMed).

Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).

Sasaki, Inoue, Katada, Taguchi: "Promotion of initial cell adhesion on trisuccinimidyl citrate-modified nickel-free high-nitrogen stainless steel." in: Journal of materials science. Materials in medicine, Vol. 24, Issue 4, pp. 951-8, (2013) (PubMed).

Yang, Cheng, Liu, Lu, Song, Jiang, Wu, Guo: "Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide) copolymer film and by trypsinization." in: Biomedical materials (Bristol, England), Vol. 7, Issue 3, pp. 35003, (2012) (PubMed).

Bai, Zeng, Zhou, Liao, Zhang, Xu, Han, Pei, Liu, Liu, Yao, Xu: "Cdc42-interacting protein-4 promotes TGF-?1-induced epithelial-mesenchymal transition and extracellular matrix deposition in renal proximal tubular epithelial cells." in: International journal of biological sciences, Vol. 8, Issue 6, pp. 859-69, (2012) (PubMed).

Mao, Gao, Li, Li: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, (2011) (PubMed).

Zeng, Han, Luo, Li, Pei, Liao, Bai, Ge, Liu, Xu: "Role of Sema4C in TGF-?1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 4, pp. 1149-56, (2011) (PubMed).

Tang, Song, Shi, Yuan, Li: "Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1." in: Biochimica et biophysica acta, Vol. 1800, Issue 3, pp. 380-4, (2010) (PubMed).

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1 (FN1 Products)

Synonyms: FN1 ELISA Kit, FN ELISA Kit, cig ELISA Kit, fibronectin ELISA Kit, finc ELISA Kit, lets ELISA Kit, msf ELISA Kit, Fn ELISA Kit, fn2 ELISA Kit, fb80d10 ELISA Kit, wu:fb80d10 ELISA Kit, CIG ELISA Kit, ED-B ELISA Kit, FINC ELISA Kit, FNZ ELISA Kit, GFND ELISA Kit, GFND2 ELISA Kit, LETS ELISA Kit, MSF ELISA Kit, E330027I09 ELISA Kit, Fn-1 ELISA Kit, FIBNEC ELISA Kit, fn-1 ELISA Kit, fibronectin 1 ELISA Kit, fibronectin 1a ELISA Kit, fibronectin 1 S homeolog ELISA Kit, FN1 ELISA Kit, fn1 ELISA Kit, fn1a ELISA Kit, Fn1 ELISA Kit, fn1.S ELISA Kit

Background:

Protein Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Background: Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Synonyms: Fibronectin,FN,Cold-insoluble globulin,CIG,Anastellin,Ugl-Y1,Ugl-Y2,Ugl-Y3,FN1,FN,

Full Gene Name: Fibronectin

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy