BMP4 ELISA Kit

Catalog No. ABIN411263

Product Details BMP4 ELISA Kit

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Binding Specificity: AA 293-408

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-4

Brand: PicoKine™

Sample Type: Osseous Tissue, Cell Culture Supernatant

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S293-R408

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Predicted Reactivity: Bovine,Monkey

Sensitivity: <4pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S293-R408

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the TGF-beta family.

Tissue Specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

Plate: Pre-coated

Protocol: human BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-4 has been precoated onto 96-well plates. Standards(NSO, S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-4 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human BMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue or cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-4 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 376, Standard deviation: 15.79, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 987, Standard deviation: 45.40, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2136, Standard deviation: 117.48, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 453, Standard deviation: 23.10, CV(%): 5.1
• Sample 2: n=24, Mean(pg/ml): 1209, Standard deviation: 68.91, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 2351, Standard deviation: 141.06, CV(%): 6.0

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP4 (BMP4 Products)

Synonyms: BMP2B ELISA Kit, BMP2B1 ELISA Kit, MCOPS6 ELISA Kit, OFC11 ELISA Kit, ZYME ELISA Kit, Bmp-4 ELISA Kit, Bmp2b ELISA Kit, Bmp2b-1 ELISA Kit, Bmp2b1 ELISA Kit, BOMPR4A ELISA Kit, bmp-4 ELISA Kit, zbmp-4 ELISA Kit, zgc:100779 ELISA Kit, BMP-4 ELISA Kit, XBMP-4 ELISA Kit, bmp2b ELISA Kit, bmp2b1 ELISA Kit, bmp4 ELISA Kit, ofc11 ELISA Kit, xbmp4 ELISA Kit, zyme ELISA Kit, BMP4 ELISA Kit, bone morphogenetic protein 4 ELISA Kit, bone morphogenetic protein 4 L homeolog ELISA Kit, bone morphogenetic protein 4 S homeolog ELISA Kit, BMP4 ELISA Kit, Bmp4 ELISA Kit, bmp4 ELISA Kit, bmp4.L ELISA Kit, bmp4.S ELISA Kit

Background:

Protein Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). .

Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

Synonyms: Bone morphogenetic protein 4,BMP-4,Bone morphogenetic protein 2B,BMP-2B,BMP4,BMP2B, DVR4,

Full Gene Name: Bone morphogenetic protein 4

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development