BMP2 ELISA Kit

Catalog No. ABIN411260

Product Details BMP2 ELISA Kit

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Binding Specificity: AA 283-396

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-2

Brand: PicoKine™

Sample Type: Osseous Tissue, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Predicted Reactivity: Bovine,Horse,Monkey,Rabbit

Sensitivity: <2pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 285, Standard deviation: 9.12, CV(%): 3.2
• Sample 2: n=16, Mean(pg/ml): 742, Standard deviation: 25.97, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 1197, Standard deviation: 37.12, CV(%): 3.1,
• Sample 1: n=24, Mean(pg/ml): 306, Standard deviation: 18.97, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 834, Standard deviation: 58.38, CV(%): 7
• Sample 3: n=24, Mean(pg/ml): 1358, Standard deviation: 97.78, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for BMP2 ELISA Kit (ABIN411260)

Jing, Hao, Xu, Liu, Xu, Luo, Meng: "Effects of local delivery of BMP2, zoledronate and their combination on bone microarchitecture, biomechanics and bone turnover in osteoporotic rabbits." in: Scientific reports, Vol. 6, pp. 28537, (2018) (PubMed).

Wang, You, Wei, Zu, Song, Li, Yan: "Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production." in: BioMed research international, Vol. 2017, pp. 2971413, (2018) (PubMed).

Li, Zhou, Wang, Yang, Ding, Zhou: "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." in: Biomaterials, Vol. 37, pp. 218-29, (2015) (PubMed).

Li, Yi, Jin, Duan, Min: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." in: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).

Han, Lin, Lu, Zhi, Wang, Meng, Jiang: "BMP2-encapsulated chitosan coatings on functionalized Ti surfaces and their performance in vitro and in vivo." in: Materials science & engineering. C, Materials for biological applications, Vol. 40, pp. 1-8, (2014) (PubMed).

Qiao, Zhang, Jin, Miao, Shi, Liu, Yuan, Liu, Li, Zheng, Zhang, Li, Yang, Sun: "Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo." in: International journal of nanomedicine, Vol. 8, pp. 2985-95, (2013) (PubMed).

Xu, Liu, Guo, Liu, Li: "Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas me?dicas e biolo?gicas / Sociedade Brasileira de Biofi?sica ... [et al.], Vol. 46, Issue 9, pp. 809-14, (2013) (PubMed).

Tian, Zhu, Qiu, Guan, Li, Zheng, Dong, Xiao: "Synthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for bone regeneration." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 32, Issue 4, pp. 563-70, (2012) (PubMed).

Ma, Ma, Guo, Zhang: "Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 85, (2010) (PubMed).

Akai, Wakisaka: "The neurohistochemistry of neuroregulators in the dental pulp." in: The Journal of Osaka University Dental School, Vol. 26, pp. 1-15, (1987) (PubMed).

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2 (BMP2 Products)

Synonyms: BDA2 ELISA Kit, BMP2A ELISA Kit, AI467020 ELISA Kit, Bmp2a ELISA Kit, BMP-2 ELISA Kit, xBMP-2 ELISA Kit, xbmp2 ELISA Kit, BMP2 ELISA Kit, bmp2a ELISA Kit, bmp2 ELISA Kit, wu:fc59d09 ELISA Kit, bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2 L homeolog ELISA Kit, Bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2a ELISA Kit, BMP2 ELISA Kit, Bmp2 ELISA Kit, bmp2.L ELISA Kit, bmp2 ELISA Kit, bmp2a ELISA Kit

Background:

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Synonyms: Bone morphogenetic protein 2 ,BMP2 ,

Full Gene Name: bone morphogenetic protein 2

Gene ID: 650

UniProt: C8C060

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation