Total Antioxidant Capacity Microplate Assay Kit
Func
Reactivity: Various Species
Colorimetric
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
Quantitative
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- Target
- Total Antioxidant Capacity
- Reactivity
- Various Species
- Detection Method
- Colorimetric
- Application
- Functional Studies (Func)
- Purpose
- Detection and Quantification of Total Antioxidant Capacity Activity.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
- Analytical Method
- Quantitative
- Characteristics
- The assay measures total antioxidant capacity in which Fe3+-TPTZ is reduced by antioxidant to Fe2+ -TPTZ. The enzyme catalysed reaction products Fe2+ -TPTZ can be measured at a colorimetric readout at 593 nm.
- Components
- 96-Well Microplate, Assay Buffer, Substrate Diluent, Substrate, Dye Reagent, Standard, Technical Manual
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Total Antioxidant Capacity ELISA Kit
Reactivity: Chemical Colorimetric Competition ELISA 21.9-4378 μmol/L
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- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Shipped and store at 4°C for 6 months.
- Expiry Date
- 6 months
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- Target
- Total Antioxidant Capacity
- Background
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Total Antioxidant Capacity, TAC, T-AOC.
An Antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline
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