Obestatin FLISA Kit
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- Target See all Obestatin (OB) FLISA Kits
- Obestatin (OB)
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Reactivity
- Rat, Mouse
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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Obestatin (OB) ELISA Kit
OB Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Obestatin (OB) ELISA KitOB Reactivity: Human Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Obestatin (OB) ELISA KitOB Reactivity: Pig Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Obestatin (OB) ELISA KitOB Reactivity: Human Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Obestatin (OB) ELISA KitOB Reactivity: Mouse Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Obestatin (OB) ELISA KitOB Reactivity: Rat Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Obestatin (OB) FLISA Kits
- Obestatin (OB)
- Alternative Name
- Obestatin (OB Products)
- Synonyms
- 2210006E23Rik FLISA Kit, Ghr FLISA Kit, MTLRP FLISA Kit, MTLRPAP FLISA Kit, m46 FLISA Kit, ghrelin FLISA Kit, Ghrl FLISA Kit
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