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Endomorphin 2 FLISA Kit

Reactivity: Human Fluorometric Competition ELISA 16.2-970 pg/mL
Catalog No. ABIN2871104
  • Target See all Endomorphin 2 FLISA Kits
    Endomorphin 2
    Reactivity
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    Human
    Detection Method
    Fluorometric
    Method Type
    Competition ELISA
    Detection Range
    16.2-970 pg/mL
    Minimum Detection Limit
    16.2 pg/mL
    Application
    ELISA
    Analytical Method
    Quantitative
    Top Product
    Discover our top product Endomorphin 2 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target See all Endomorphin 2 FLISA Kits
    Endomorphin 2
    Alternative Name
    Endomorphin-2 (Endomorphin 2 Products)
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