Angiotensin II FLISA Kit
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- Target See all Angiotensin II (Ang II) FLISA Kits
- Angiotensin II (Ang II)
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Reactivity
- Human, Rat, Mouse, Dog, Pig, Guinea Pig
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Featured
- Discover our best selling Ang II ELISA Kit
- Top Product
- Discover our top product Ang II ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Angiotensin II (Ang II) FLISA Kits
- Angiotensin II (Ang II)
- Alternative Name
- Angiotensin II (Ang II Products)
- Synonyms
- ANHU FLISA Kit, SERPINA8 FLISA Kit, ANRT FLISA Kit, Ang FLISA Kit, AngII FLISA Kit, PAT FLISA Kit, AI265500 FLISA Kit, AngI FLISA Kit, Aogen FLISA Kit, Serpina8 FLISA Kit, angiotensinogen FLISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) FLISA Kit, AGT FLISA Kit, Agt FLISA Kit
- Pathways
- JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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