FOSB ELISA Kit

Catalog No. ABIN2866257

Product Details FOSB ELISA Kit

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Brand: TransAM®

Sample Type: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificity: TransAM AP-1 FosB Kits are tested for sensitivity in detecting FosB in nuclear extracts from K-562 cells that are either unstimulated or stimulated with TPA or TPA/Ionomycin.

Characteristics: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Components: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

These extracts are diluted down to 1.25 μg/well and assayed using the TransAM AP-1 FosB Kit. The ratio of the signals from the stimulated over the unstimulated cells must be above 4. Lot No. 00412005 was tested using K-562 cells. It was developed for 3 minutes and gave a ratio of 7 (Figure 1). The basal level of FosB expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AP-1 FosB Kits are also tested for specificity in detecting FosB activation. TransAM AP-1 FosB assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AP-1 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AP-1 binding.

Assay Time: 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, poly[d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for FOSB

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Alternative Name: Ap-1 Fosb (FOSB Products)

Synonyms: FOSB ELISA Kit, zgc:92354 ELISA Kit, AP-1 ELISA Kit, G0S3 ELISA Kit, GOS3 ELISA Kit, GOSB ELISA Kit, fra-2 ELISA Kit, FBJ murine osteosarcoma viral oncogene homolog B ELISA Kit, FosB proto-oncogene, AP-1 transcription factor subunit ELISA Kit, FBJ osteosarcoma oncogene B ELISA Kit, FOSB ELISA Kit, fosb ELISA Kit, Fosb ELISA Kit