c-FOS ELISA Kit

Catalog No. ABIN2866254

Product Details c-FOS ELISA Kit

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Brand: TransAM®

Sample Type: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificity: TransAM AP-1 c-Fos Kits are tested for sensitivity in detecting c-Fos in nuclear extracts from K-562 or WI-38 VA13 cells that are either unstimulated or stimulated with TPA or TPA/Ionomycin.

Characteristics: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Components: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

These extracts are diluted down to 0.625 μg/well and assayed using the TransAM AP-1 c-Fos Kit. The ratio of the signals from the stimulated over the unstimulated cells must be above 2. Lot No. 00412017 using K-562 cells was developed for 2 minutes. It gave a ratio of 26 (Figure 1). The basal level of c-Fos expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AP-1 c-Fos Kits are also tested for specificity in detecting c-Fos activation. TransAM AP-1 c-Fos assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AP-1 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AP-1 binding.

Assay Time: 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: -20 °C/-80 °C

Storage Comment: Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. This product is guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: Ap-1 C-Fos (c-Fos Products)

Synonyms: AP-1 ELISA Kit, C-FOS ELISA Kit, p55 ELISA Kit, c-fos ELISA Kit, D12Rfj1 ELISA Kit, cFos ELISA Kit, cFOS ELISA Kit, fos ELISA Kit, Fos proto-oncogene, AP-1 transcription factor subunit ELISA Kit, FBJ osteosarcoma oncogene ELISA Kit, proto-oncogene c-Fos-like ELISA Kit, FOS ELISA Kit, Fos ELISA Kit, LOC101078177 ELISA Kit

Pathways: S100 Proteins