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Histone 3 ELISA Kit

H3 Reactivity: Human H3K4me Colorimetric Sandwich ELISA
Catalog No. ABIN2866239
  • Target See all Histone 3 (H3) ELISA Kits
    Histone 3 (H3)
    Binding Specificity
    H3K4me
    Reactivity
    • 20
    • 10
    • 7
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Histone H3 methylated Lys4 ELISA to quantitate the level of histone H3 lysine 4 methylation
    Analytical Method
    Quantitative
    Specificity
    Histone H3 monomethyl Lys4 ELISA Kits are tested for sensitivity in detecting monomethylated lysine 4 on histone H3 by generating a standard curve using the Recombinant Histone H3 monomethyl Lys4 protein provided in the kit.
    Components
    Histone H3 monomethyl Lys4 ELISA Kits include a 96-stripwell Histone H3 Capture plate, a primary antibody specific for Histone H3 monomethyl Lys4, HRP-conjugated anti-rabbit IgG secondary antibody, Assay Dilution Buffer, 20X Wash Buffer, Developing Solution, Stop Solution, Recombinant Histone H3 monomethyl Lys4 positive control protein and plate sealer. 
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Recombinant protein standards are generated in duplicate from 3.9 ng - 250 ng and assayed in the kit according to the protocol listed in the manual. For lot 08810001, a developing time of 5 minutes generated the protein standard curve in Figure 1. The linear range of this lot was plotted in Figure 2. These results are provided for demonstration only. The linear range may vary depending on the developing time used and should be calculated each time a standard curve is run.

    Assay Time
    3 h
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C/-80 °C
    Storage Comment
    Store the Recombinant Histone H3 monomethyl Lys4 protein at -80°C. All other components should be stored at 4°C. This product is guaranteed for 6 months from date of receipt.
    Expiry Date
    6 months
  • Target See all Histone 3 (H3) ELISA Kits
    Histone 3 (H3)
    Alternative Name
    Histone h3 (H3 Products)
    Synonyms
    H-3 ELISA Kit, histocompatibility 3 ELISA Kit, H3 ELISA Kit
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