Rapid Mouse Monoclonal Antibody Isotyping Kit

Catalog No. ABIN2860627

Product Details

Reactivity: Mouse

Minimum Detection Limit: 100 ng/mL

Application: Isotyping (IsoT)

Purpose: 5 minute determination of 6 mouse Ig heavy chain isotypes and 2 light chain subclasses

Sample Type: Cell Culture Supernatant, Purified Protein

Cross-Reactivity (Details): There is no cross-reactivity among each isotype.

Characteristics: The Rapid Mouse Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination.
Acceptable sample types include hybridoma culture supernatant and purified monoclonal antibody.
Ascites fluid is not recommended since it usually contains multiple immunoglobulins.
However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid.

Components: The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2b, IgG2c (*) and IgG3, and the other cassette is for determining κ, λ, IgA and IgM.
Both strips contain a control line.

Application Details

Comment:

1. Invalid result: no red line at ""C"".
2. Negative result: only one red line at ""C"".
3. Positive results:
a). Single monoclonal antibody: In addition to the positive control ""C"" line, only one heavy chain and one light chain will appear.
b). Multiple monoclonal antibodies: In addition to the positive control line, more than one heavy chain and/or one light chain will appear.
The line intensity can be used to judge the dominant Isotypes.

Sample Volume: 100 µL

Assay Time: 5 min

Protocol: 1) Place the cassettes in horizontal plane.
2) Add 80-100 μL antibody solution directly to the Sample Well. Wait for 5 minutes.
3) A red test line at the location corresponding to antibody-specific isotype and a red control line at ""C' position will appear within 1-5 minutes.
NOTE: Do not read after 20 minutes.
NOTE: If the color of tissue culture medium causes high background, 40-60 μL PBS can be chased to clear the background.

Sample Preparation:

Cell Culture Supernatant: Recommended dilution is 1:10-1:100 in PBS.
Purified Antibody: Dilute samples to 0.5 - 2 μg/mL in PBS
Ascites: Ascites fluid is not recommended since it usually contains multiple Igs. However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid. The recommended dilution is 1:10,000 in PBS.

Restrictions: For Research Use only

Handling

Handling Advice: Promptly return unused strips in sealed bag and keep dry.

Storage: RT

Storage Comment: Store the strips in their original package at room temperature.
This product is stable for up to 18 months from the certified date. Freezing the strips is not recommended.

Expiry Date: 18 months