DKK3 ELISA Kit

Catalog No. ABIN2859331

Product Details DKK3 ELISA Kit

Target: DKK3

Binding Specificity: AA 22-350

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-3

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A22-I350

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A22-I350

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Highest expression in heart, brain, and spinal cord. .

Plate: Pre-coated

Protocol: human DKK-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK-3 has been precoated onto 96-well plates. Standards(NSO, A22-I350) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK-3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human DKK-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DKK-3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1228, Standard deviation: 79.82, CV(%): 6.5
• Sample 2: n=16, Mean(pg/ml): 3386, Standard deviation: 169.3, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 5833, Standard deviation: 332.5, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1439, Standard deviation: 110.8, CV(%): 7.7
• Sample 2: n=24, Mean(pg/ml): 3627, Standard deviation: 196, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 6294, Standard deviation: 428, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for DKK3

Target: DKK3

Abstract: DKK3 Products

Synonyms: REIC ELISA Kit, RIG ELISA Kit, reic ELISA Kit, dkk-3 ELISA Kit, AW061014 ELISA Kit, C87148 ELISA Kit, Reic ELISA Kit, p29 ELISA Kit, dickkopf WNT signaling pathway inhibitor 3 ELISA Kit, dickkopf WNT signaling pathway inhibitor 3 S homeolog ELISA Kit, DKK3 ELISA Kit, dkk3 ELISA Kit, dkk3.S ELISA Kit, Dkk3 ELISA Kit

Background:

Protein Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). .

Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Synonyms: Dickkopf-related protein 3,Dickkopf-3,Dkk-3,hDkk-3,DKK3,REIC,UNQ258/PRO295,

Full Gene Name: Dickkopf-related protein 3

Cellular Localisation: Secreted.

Gene ID: 27122

UniProt: Q9UBP4