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Relaxin 1 ELISA Kit

RLN1 Reactivity: Human AA 26-53 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Catalog No. ABIN2859323
  • Target See all Relaxin 1 (RLN1) ELISA Kits
    Relaxin 1 (RLN1)
    Binding Specificity
    AA 26-53
    Reactivity
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78-5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Relaxin 1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificity
    E.coli, R162-Cys185(A)&K26-S53(B)
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: R162-Cys185(A)&K26-S53(B)
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Prostate. Not expressed in placenta, decidua or ovary. .

    Plate
    Pre-coated
    Protocol
    human Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli,R162-Cys185 (A) & K26-S53 (B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 1 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Relaxin 1 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 596, Standard deviation: 29.20, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 1354, Standard deviation: 69.05, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 2415, Standard deviation: 130.41, CV(%): 5.4,
    • Sample 1: n=24, Mean(pg/ml): 688, Standard deviation: 35.77, CV(%): 5.2
    • Sample 2: n=24, Mean(pg/ml): 1542, Standard deviation: 92.52, CV(%): 6
    • Sample 3: n=24, Mean(pg/ml): 2229, Standard deviation: 151.57, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Relaxin 1 (RLN1) ELISA Kits
    Relaxin 1 (RLN1)
    Alternative Name
    RLN1 (RLN1 Products)
    Synonyms
    H1 ELISA Kit, H1RLX ELISA Kit, RLXH1 ELISA Kit, bA12D24.3.1 ELISA Kit, bA12D24.3.2 ELISA Kit, Rln ELISA Kit, rlx ELISA Kit, RELAX ELISA Kit, RLX1 ELISA Kit, RNL1 ELISA Kit, RLN1 ELISA Kit, RLX ELISA Kit, relaxin 1 ELISA Kit, RLN1 ELISA Kit, Rln1 ELISA Kit
    Background

    Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

    Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

    Synonyms: Prorelaxin H1,Relaxin B chain,Relaxin A chain,RLN1,

    Full Gene Name: Prorelaxin H1

    Cellular Localisation: Secreted.
    Gene ID
    6013
    UniProt
    P04808
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