IL17C ELISA Kit

Catalog No. ABIN2859298

Product Details IL17C ELISA Kit

Target: IL17C (Interleukin 17C (IL17C))

Binding Specificity: AA 17-194

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125-8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17C

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: D17-Q194

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: D17-Q194

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-17 family.

Tissue Specificity: Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes. .

Plate: Pre-coated

Protocol: mouse IL-17C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli, D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17C amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL-17C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17C standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.3, Standard deviation: 0.053, CV(%): 4.1
• Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.186, CV(%): 4.9
• Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.296, CV(%): 5.2,
• Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.086, CV(%): 5.4
• Sample 2: n=24, Mean(ng/ml): 4.5, Standard deviation: 0.279, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 6.3, Standard deviation: 0.422, CV(%): 6.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for IL17C

Target: IL17C (Interleukin 17C (IL17C))

Alternative Name: IL17C (IL17C Products)

Synonyms: IL17C ELISA Kit, il17-3 ELISA Kit, CX2 ELISA Kit, IL-17C ELISA Kit, IL-21 ELISA Kit, interleukin 17C ELISA Kit, interleukin 17c ELISA Kit, IL17C ELISA Kit, il17c ELISA Kit, Il17c ELISA Kit

Background:

Protein Function: Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis. .

Background: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Synonyms: Interleukin-17C,Il-17c,Cytokine CX2,Il17c,

Full Gene Name: Interleukin-17C

Cellular Localisation: Secreted.

Gene ID: 234836

UniProt: Q8K4C5

Pathways: Cellular Response to Molecule of Bacterial Origin