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IL17 Receptor B ELISA Kit

IL17RB Reactivity: Human AA 18-286 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859296
  • Target See all IL17 Receptor B (IL17RB) ELISA Kits
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Binding Specificity
    AA 18-286
    Reactivity
    • 7
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17RB
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: R18-K286
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: R18-K286
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 SEFIR domain.

    Tissue Specificity: Expressed in several endocrine tissues, mostly in fetal and adult liver, kidney, pancreas, testis, colon, brain and small intestine, not detected in peripheral blood leukocytes, lymphoid organs, and most cell lines.

    Plate
    Pre-coated
    Protocol
    human IL-17RB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RB has been precoated onto 96-well plates. Standards(NSO, R18-K286) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RB amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-17RB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-17RB standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.43, Standard deviation: 0.053, CV(%): 3.7
    • Sample 2: n=16, Mean(ng/ml): 3.04, Standard deviation: 0.137, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 6.12, Standard deviation: 0.324, CV(%): 5.3,
    • Sample 1: n=24, Mean(ng/ml): 1.86, Standard deviation: 0.106, CV(%): 5.7
    • Sample 2: n=24, Mean(ng/ml): 3.43, Standard deviation: 0.233, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 6.51, Standard deviation: 0.469, CV(%): 7.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IL17 Receptor B (IL17RB) ELISA Kits
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Alternative Name
    IL17RB (IL17RB Products)
    Synonyms
    IL17RB ELISA Kit, CRL4 ELISA Kit, EVI27 ELISA Kit, IL17BR ELISA Kit, IL17RH1 ELISA Kit, Evi27 ELISA Kit, IL-17ER ELISA Kit, IL-17Rh1 ELISA Kit, Il17br ELISA Kit, interleukin 17 receptor B ELISA Kit, IL17RB ELISA Kit, Il17rb ELISA Kit
    Background

    Protein Function: Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. .

    Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.

    Synonyms: Interleukin-17 receptor B,IL-17 receptor B,IL-17RB,Cytokine receptor-like 4,IL-17 receptor homolog 1,IL-17Rh1,IL17Rh1,Interleukin-17B receptor,IL-17B receptor,IL17RB,CRL4, EVI27, IL17BR,UNQ2501/PRO19612,

    Full Gene Name: Interleukin-17 receptor B

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    55540
    UniProt
    Q9NRM6
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