NRP2 ELISA Kit

Catalog No. ABIN2859218

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat NRP2.

Quick Overview for NRP2 ELISA Kit (ABIN2859218)

Target: NRP2 (Neuropilin 2 (NRP2))

Binding Specificity: AA 23-857

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Product Details NRP2 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Neuropilin-2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q23-D857

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q23-D857

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the neuropilin family.

Tissue Specificity: Found in certain neuronal populations of the CNS and in other non-neuronal tissues including mesenchymal tissue lining in the ribs.

Plate: Pre-coated

Protocol: rat Neuropilin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(NSO, Q23-D857) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neuropilin-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Neuropilin-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.6, Standard deviation: 0.058, CV(%): 3.6
• Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.161, CV(%): 4.6
• Sample 3: n=16, Mean(ng/ml): 6.1, Standard deviation: 0.317, CV(%): 5.2,
• Sample 1: n=24, Mean(ng/ml): 1.9, Standard deviation: 0.089, CV(%): 4.7
• Sample 2: n=24, Mean(ng/ml): 4.2, Standard deviation: 0.231, CV(%): 5.5
• Sample 3: n=24, Mean(ng/ml): 7.3, Standard deviation: 0.467, CV(%): 6.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for NRP2

Target: NRP2 (Neuropilin 2 (NRP2))

Alternative Name: NRP2

Background:

Protein Function: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Synonyms: Neuropilin-2,Vascular endothelial cell growth factor 165 receptor 2,Nrp2,

Full Gene Name: Neuropilin-2

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 81527

UniProt: O35276

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals