NRP2 ELISA Kit

Catalog No. ABIN2859217

Product Details NRP2 ELISA Kit

Target: NRP2 (Neuropilin 2 (NRP2))

Binding Specificity: AA 23-863

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neuropilin-2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q23-D863

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q23-D863

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the neuropilin family.

Tissue Specificity: Expressed in developing CNS, PNS and in some nonneural tissues including limb buds, developing bones, muscles, intestinal epithelium, kidney, lung and submandibular gland.

Plate: Pre-coated

Protocol: mouse Neuropilin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(NSO, Q23-D863) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neuropilin-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Neuropilin-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1590, Standard deviation: 68.37, CV(%): 4.3
• Sample 2: n=16, Mean(pg/ml): 3425, Standard deviation: 178.1, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 6308, Standard deviation: 359.6, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1736, Standard deviation: 95.48, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 3621, Standard deviation: 231.7, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 6738, Standard deviation: 464.9, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for NRP2

Target: NRP2 (Neuropilin 2 (NRP2))

Alternative Name: NRP2 (NRP2 Products)

Synonyms: NP2 ELISA Kit, NPN2 ELISA Kit, PRO2714 ELISA Kit, VEGF165R2 ELISA Kit, 1110048P06Rik ELISA Kit, Np-2 ELISA Kit, Np2 ELISA Kit, Npn-2 ELISA Kit, Npn2 ELISA Kit, neuropilin-2 ELISA Kit, neuropilin 2 ELISA Kit, NRP2 ELISA Kit, Nrp2 ELISA Kit

Background:

Protein Function: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Synonyms: Neuropilin-2,Vascular endothelial cell growth factor 165 receptor 2,Nrp2,

Full Gene Name: Neuropilin-2

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 18187

UniProt: O35375

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals