RPS6 ELISA Kit

Catalog No. ABIN2748616

Product Details RPS6 ELISA Kit

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Binding Specificity: pSer235, pSer236, total

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human/Mouse/Rat Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit. This assay semi-quantitatively measures phophorylated RPS6 (Ser235/S236) and Total RPS6 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-RPS6 (pSer235/Ser236) and total RPS6.

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6 (RPS6 Products)

Synonyms: S6 ELISA Kit, rps6 ELISA Kit, rps6a ELISA Kit, S6R ELISA Kit, (Rp)S6 ELISA Kit, CG10944 ELISA Kit, DS6 ELISA Kit, Dmel\\CG10944 ELISA Kit, M(1)7BC ELISA Kit, M(1)7C ELISA Kit, RPS6 ELISA Kit, Rp S6 ELISA Kit, Rps6 ELISA Kit, air8 ELISA Kit, air[8] ELISA Kit, anon-WO02059370.61 ELISA Kit, hen ELISA Kit, l(1)air8 ELISA Kit, l(1)air[8] ELISA Kit, l(1)hen ELISA Kit, pp30 ELISA Kit, rpS6 ELISA Kit, wu:fa92e06 ELISA Kit, wu:fb64g06 ELISA Kit, zgc:92237 ELISA Kit, ribosomal protein S6 ELISA Kit, ribosomal protein S6 S homeolog ELISA Kit, S6 ribosomal protein ELISA Kit, Ribosomal protein S6 ELISA Kit, 30S ribosomal protein S6 ELISA Kit, 40S ribosomal protein S6 ELISA Kit, RPS6 ELISA Kit, rps6.S ELISA Kit, LOC100135859 ELISA Kit, Rps6 ELISA Kit, RpS6 ELISA Kit, rps6 ELISA Kit, rps-6 ELISA Kit, LOC100533219 ELISA Kit

Background: Ribosomal protein S6 phosphorylated at S235/S236 and total RPS6

Gene ID: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly