PDGFRA ELISA Kit
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- Target See all PDGFRA ELISA Kits
- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
- Binding Specificity
- pTyr
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human Phosphotyrosine PDGFRA ELISA Kit. This assay semi-quantitatively measures phosphotyrosine PDGFRA in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-PDGFRA.
- Characteristics
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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- Target See all PDGFRA ELISA Kits
- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
- Alternative Name
- PDGFRA (PDGFRA Products)
- Synonyms
- AI115593 ELISA Kit, CD140a ELISA Kit, Pdgfr-2 ELISA Kit, APDGFR ELISA Kit, PDGFACE ELISA Kit, CD140A ELISA Kit, PDGFR-2 ELISA Kit, PDGFR2 ELISA Kit, RHEPDGFRA ELISA Kit, PDGFR ELISA Kit, PDGFR-A ELISA Kit, platelet derived growth factor receptor alpha ELISA Kit, platelet derived growth factor receptor, alpha polypeptide ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide L homeolog ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide ELISA Kit, PDGFRA ELISA Kit, Pdgfra ELISA Kit, pdgfra.L ELISA Kit
- Background
- Platelet-Derived Growth Factor Receptor, Alpha Polypeptide, PDGFR2, Alpha-Type Platelet-Derived Growth Factor Receptor, Platelet-Derived Growth Factor Receptor 2, PDGF-R-Alpha, PDGFR-2, PDGFR-Alpha, RHEPDGFRA, CD140 Antigen-Like Family Member A, CD140a Antigen, CD140a, CD140A, PDGFRA/BCR Fusion, Platelet-Derived Growth Factor Receptor Alpha, Rearranged-In-Hypereosinophilia-Platelet Derived Growth Factor Receptor AlphaFusion Protein, Alpha Platelet-Derived Growth Factor Receptor, Platelet-Derived Growth Factor Alpha Receptor
- Gene ID
- 5156
- UniProt
- P16234
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
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