CDKN1B ELISA Kit

Catalog No. ABIN2748475

Product Details CDKN1B ELISA Kit

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Mouse p27/Kip1 (CDKN1B) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse p27/Kip1 (CDKN1B)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: p27/Kip1 (CDKN1B Products)

Synonyms: CDKN4 ELISA Kit, KIP1 ELISA Kit, MEN1B ELISA Kit, MEN4 ELISA Kit, P27KIP1 ELISA Kit, cdkn1b ELISA Kit, sb:cb611 ELISA Kit, wu:fb63g10 ELISA Kit, wu:fb64g10 ELISA Kit, wu:fe18e03 ELISA Kit, CDKN1B ELISA Kit, Kip1 ELISA Kit, p27 ELISA Kit, FAM14D ELISA Kit, ISG12 ELISA Kit, ISG12A ELISA Kit, P27 ELISA Kit, Cdki1b ELISA Kit, AA408329 ELISA Kit, AI843786 ELISA Kit, p27Kip1 ELISA Kit, cdkn1bl ELISA Kit, cyclin dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Bb ELISA Kit, interferon alpha inducible protein 27 ELISA Kit, cyclin-dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Ba ELISA Kit, CDKN1B ELISA Kit, cdkn1bb ELISA Kit, IFI27 ELISA Kit, Cdkn1b ELISA Kit, cdkn1ba ELISA Kit

Background: P27/Kip1 (CDKN1B)

Gene ID: 821314

UniProt: Q43735

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy