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NOTCH2 ELISA Kit

NOTCH2 Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2748445
  • Target See all NOTCH2 ELISA Kits
    NOTCH2 (Notch 2 (NOTCH2))
    Reactivity
    • 3
    • 2
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rat Notch-2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Rat Notch-2
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all NOTCH2 ELISA Kits
    NOTCH2 (Notch 2 (NOTCH2))
    Alternative Name
    Notch-2 (NOTCH2 Products)
    Synonyms
    AGS2 ELISA Kit, HJCYS ELISA Kit, hN2 ELISA Kit, AI853703 ELISA Kit, N2 ELISA Kit, notch6 ELISA Kit, sb:cb884 ELISA Kit, neurogenic locus notch homolog protein 2 ELISA Kit, notch 2 ELISA Kit, notch 3 ELISA Kit, LOC581186 ELISA Kit, NOTCH2 ELISA Kit, notch3 ELISA Kit, Notch2 ELISA Kit, notch2 ELISA Kit
    Background
    Notch-2
    Gene ID
    29492
    UniProt
    Q9QW30
    Pathways
    Notch Signaling, Stem Cell Maintenance
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