NOTCH2 ELISA Kit
-
- Target See all NOTCH2 ELISA Kits
- NOTCH2 (Notch 2 (NOTCH2))
-
Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Rat Notch-2 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Rat Notch-2
- Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling NOTCH2 ELISA Kit
- Top Product
- Discover our top product NOTCH2 ELISA Kit
-
-
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
-
Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
-
- Target See all NOTCH2 ELISA Kits
- NOTCH2 (Notch 2 (NOTCH2))
- Alternative Name
- Notch-2 (NOTCH2 Products)
- Synonyms
- AGS2 ELISA Kit, HJCYS ELISA Kit, hN2 ELISA Kit, AI853703 ELISA Kit, N2 ELISA Kit, notch6 ELISA Kit, sb:cb884 ELISA Kit, neurogenic locus notch homolog protein 2 ELISA Kit, notch 2 ELISA Kit, notch 3 ELISA Kit, LOC581186 ELISA Kit, NOTCH2 ELISA Kit, notch3 ELISA Kit, Notch2 ELISA Kit, notch2 ELISA Kit
- Background
- Notch-2
- Gene ID
- 29492
- UniProt
- Q9QW30
- Pathways
- Notch Signaling, Stem Cell Maintenance
-