MTOR ELISA Kit
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- Target See all MTOR (mTOR) ELISA Kits
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Binding Specificity
- pSer2448
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human Phospho-mTOR (S2448) ELISA Kit. This assay semi-quantitatively measures phophorylated mTOR (Ser2448) in lysate samples.
- Sample Type
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificity
- The antibody pair provided in this kit recognizes human mTOR phosphorylated at site Serine-2448.
- Characteristics
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Material not included
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Expiry Date
- 6 months
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- Target See all MTOR (mTOR) ELISA Kits
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Alternative Name
- mTOR (mTOR Products)
- Synonyms
- FRAP1 ELISA Kit, FRAP ELISA Kit, FRAP2 ELISA Kit, RAFT1 ELISA Kit, RAPT1 ELISA Kit, 2610315D21Rik ELISA Kit, AI327068 ELISA Kit, Frap1 ELISA Kit, flat ELISA Kit, frap1 ELISA Kit, tor ELISA Kit, wu:fc22h08 ELISA Kit, mechanistic target of rapamycin kinase ELISA Kit, mechanistic target of rapamycin ELISA Kit, mechanistic target of rapamycin (serine/threonine kinase) ELISA Kit, MTOR ELISA Kit, Mtor ELISA Kit, mtor ELISA Kit
- Background
- Mechanistic Target Of Rapamycin (mTOR) serine/threonine kinase - phosphorylated at Ser2448
- Gene ID
- 2475
- UniProt
- P42345
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
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