IL-1 beta ELISA Kit
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- Target See all IL-1 beta (IL1B) ELISA Kits
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Reactivity
- Rabbit
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 45-12.000 pg/mL
- Minimum Detection Limit
- 45 pg/mL
- Application
- ELISA
- Purpose
- Rabbit IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rabbit IL-1alpha, IL-8, IL-17A, IL-21, Leptin, MIP-1 beta, MMP-9, NCAM-1, TNF alpha
- Sensitivity
- 45 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Recommended Dilution for serum and plasma samples2 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries." in: Stem cell research & therapy, Vol. 6, pp. 173, (2015) (PubMed).
: "
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The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries." in: Stem cell research & therapy, Vol. 6, pp. 173, (2015) (PubMed).
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- Target See all IL-1 beta (IL1B) ELISA Kits
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Alternative Name
- IL-1 beta (IL1B Products)
- Synonyms
- IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit
- Background
- Interleukin-1 beta (IL-1 beta) (Lymphocyte proliferation-potentiating factor)
- Gene ID
- 100008990
- UniProt
- P14628
- Pathways
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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