IGF1R ELISA Kit

Catalog No. ABIN2748188

Product Details IGF1R ELISA Kit

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Binding Specificity: pTyr1165, pTyr1166

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phospho-IGF1R (Y1165/1166) Kit. This assay semi-quantitatively measures phophorylated IGF1R (Tyr1165/1166) in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human IGF1R phosphorylated at site Tyrosine-1165/1166.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R (IGF1R Products)

Synonyms: CMT2N ELISA Kit, CD221 ELISA Kit, IGFIR ELISA Kit, IGFR ELISA Kit, JTK13 ELISA Kit, IGFIRC ELISA Kit, IGFR1 ELISA Kit, IGF-1R ELISA Kit, IGF1R ELISA Kit, DKFZp469I0618 ELISA Kit, igf-1r ELISA Kit, xIGF-1R ELISA Kit, xIGFR ELISA Kit, xigf1r ELISA Kit, A330103N21Rik ELISA Kit, D930020L01 ELISA Kit, hyft ELISA Kit, igf-ir ELISA Kit, IGF-IR ELISA Kit, IGF-IRb ELISA Kit, wu:fb03h06 ELISA Kit, IGF-IRa ELISA Kit, sb:eu1090 ELISA Kit, wu:fi20b10 ELISA Kit, alanyl-tRNA synthetase ELISA Kit, insulin like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 ELISA Kit, insulin-like growth factor I receptor ELISA Kit, insulin like growth factor 1 receptor S homeolog ELISA Kit, insulin-like growth factor 1b receptor ELISA Kit, insulin-like growth factor 1a receptor ELISA Kit, AARS ELISA Kit, IGF1R ELISA Kit, Igf1r ELISA Kit, igf1r ELISA Kit, Igf1 ELISA Kit, LOC780503 ELISA Kit, LOC100136427 ELISA Kit, igf1r.S ELISA Kit, igf1rb ELISA Kit, igf1ra ELISA Kit

Background: Insulin-like growth factor 1 receptor (IGF1R) phosphorylated at Tyrosine-1165/1166

Gene ID: 3480

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy