FAP ELISA Kit

Catalog No. ABIN2748101

Product Details FAP ELISA Kit

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Rhesus Monkey

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Rhesus Macaque Fibroblast Activation Protein (FAP) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Rhesus Macaque Fibroblast Activation Protein (FAP)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP (FAP Products)

Synonyms: fap-A ELISA Kit, gene 13 ELISA Kit, FAP ELISA Kit, fapa ELISA Kit, dppiv ELISA Kit, seprase ELISA Kit, DPPIV ELISA Kit, FAPA ELISA Kit, fibroblast activation protein ELISA Kit, fibroblast activation protein alpha L homeolog ELISA Kit, fibroblast activation protein alpha ELISA Kit, fibroblast activation protein, alpha ELISA Kit, prolyl endopeptidase FAP ELISA Kit, Fap ELISA Kit, fap.L ELISA Kit, FAP ELISA Kit, fap ELISA Kit, LOC100547273 ELISA Kit

Background: Fibroblast Activation Protein (FAP)

Gene ID: 701541

UniProt: F7EF58

Pathways: Tube Formation