DLL1 ELISA Kit

Catalog No. ABIN2748051

Product Details DLL1 ELISA Kit

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Rat DLL1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Rat DLL1. Other species not yet determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1 (DLL1 Products)

Synonyms: X-delta-1 ELISA Kit, XDelta1 ELISA Kit, Xdelta-1 ELISA Kit, delta ELISA Kit, delta-1 ELISA Kit, delta1 ELISA Kit, x-delta ELISA Kit, DELTA1 ELISA Kit, DL1 ELISA Kit, Delta ELISA Kit, DLK ELISA Kit, DLK-1 ELISA Kit, Delta1 ELISA Kit, FA1 ELISA Kit, PREF1 ELISA Kit, Pref-1 ELISA Kit, ZOG ELISA Kit, pG2 ELISA Kit, DLL1 ELISA Kit, AW742678 ELISA Kit, DlkI ELISA Kit, Ly107 ELISA Kit, Peg9 ELISA Kit, SCP1 ELISA Kit, pref-1 ELISA Kit, delta-like 1 ELISA Kit, delta like canonical Notch ligand 1 ELISA Kit, delta like non-canonical Notch ligand 1 ELISA Kit, delta-like 1 L homeolog ELISA Kit, delta-like 1 (Drosophila) ELISA Kit, delta-like 1 homolog (Drosophila) ELISA Kit, dll1 ELISA Kit, DLL1 ELISA Kit, DLK1 ELISA Kit, Dll1 ELISA Kit, dll1.L ELISA Kit, Dlk1 ELISA Kit

Background: Delta-like protein 1 (Drosophila Delta homolog 1) (Delta1)

Gene ID: 84010

UniProt: P97677

Pathways: Notch Signaling, Stem Cell Maintenance