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BCAM ELISA Kit

BCAM Reactivity: Rhesus Monkey Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2747976
  • Target See all BCAM ELISA Kits
    BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))
    Reactivity
    • 5
    • 2
    • 1
    • 1
    • 1
    Rhesus Monkey
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rhesus Macaque Basal Cell Adhesion Molecule (BCAM) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Rhesus Macaque Basal Cell Adhesion Molecule (BCAM)
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product BCAM ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all BCAM ELISA Kits
    BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))
    Alternative Name
    BCAM (BCAM Products)
    Synonyms
    cd239 ELISA Kit, msk19 ELISA Kit, MGC81012 ELISA Kit, LU ELISA Kit, prr2 ELISA Kit, zgc:154034 ELISA Kit, 1200005K12Rik ELISA Kit, B-CAM ELISA Kit, Gplu ELISA Kit, Lu ELISA Kit, AU ELISA Kit, CD239 ELISA Kit, MSK19 ELISA Kit, basal cell adhesion molecule (Lutheran blood group) ELISA Kit, basal cell adhesion molecule (Lutheran blood group) L homeolog ELISA Kit, basal cell adhesion molecule ELISA Kit, bcam ELISA Kit, bcam.L ELISA Kit, BCAM ELISA Kit, Bcam ELISA Kit
    Background
    Basal Cell Adhesion Molecule (BCAM)
    UniProt
    H9Z2N4
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