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HAVCR1 ELISA Kit
HAVCR1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
30-8.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details HAVCR1 ELISA Kit
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Target
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HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
Reactivity
All reactivities for HAVCR1 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
30-8.000 pg/mL
Minimum Detection Limit
30 pg/mL
Application
ELISA
Purpose
Mouse TIM-1 (KIM-1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-2, TIM-3, TRAIL, Tryptase epsilon
Sensitivity
30 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for HAVCR1
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Target
See all HAVCR1 ELISA Kits
HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
Alternative Name
TIM-1 (HAVCR1 Products )
Synonyms
HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit
Target Type
Virus
Background
Gene Names: Havcr1 Kim1 Tim1 Timd1 Protein names: Hepatitis A virus cellular receptor 1 homolog (HAVcr-1) (Kidney injury molecule 1) (KIM-1) (T cell immunoglobulin and mucin domain-containing protein 1) (TIMD-1) (T cell membrane protein 1) (T-cell immunoglobulin mucin receptor 1) (TIM-1)
Gene ID
171283
UniProt
Q5QNS5
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