Thrombospondin 1 ELISA Kit

Catalog No. ABIN2703490

Product Details Thrombospondin 1 ELISA Kit

Target: Thrombospondin 1 (THBS1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-2000 ng/mL

Minimum Detection Limit: 6 ng/mL

Application: ELISA

Purpose: Human Thrombospondin 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Thrombospondin-1. It shows less than 5 % cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.

Sensitivity: 6 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples10 - 200 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: Thrombospondin 1 (THBS1 Products)

Synonyms: THBS ELISA Kit, THBS-1 ELISA Kit, TSP ELISA Kit, TSP-1 ELISA Kit, TSP1 ELISA Kit, Thbs-1 ELISA Kit, tbsp1 ELISA Kit, thrombospondin-1 ELISA Kit, thbs ELISA Kit, thbs-1 ELISA Kit, tsp ELISA Kit, tsp-1 ELISA Kit, tsp1 ELISA Kit, Tsp1 ELISA Kit, THBS1 ELISA Kit, Tsp-1 ELISA Kit, wu:fc48c03 ELISA Kit, si:dkey-11e23.1 ELISA Kit, tsp2 ELISA Kit, thrombospondin 1 ELISA Kit, thrombospondin 1 S homeolog ELISA Kit, thrombospondin 1b ELISA Kit, thrombospondin 2 L homeolog ELISA Kit, THBS1 ELISA Kit, Thbs1 ELISA Kit, thbs1.S ELISA Kit, thbs1b ELISA Kit, thbs1 ELISA Kit, thbs2.L ELISA Kit

Background: Gene Names: THBS1 TSP TSP1
Protein names: Thrombospondin-1

Gene ID: 7057

UniProt: P07996

Pathways: Autophagy