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tau ELISA Kit

Reactivity: Human Colorimetric Sandwich ELISA 100-25.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2703476
  • Target See all tau ELISA Kits
    tau (tau Protein)
    Reactivity
    • 19
    • 19
    • 17
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    100-25.000 pg/mL
    Minimum Detection Limit
    100 pg/mL
    Application
    ELISA
    Purpose
    Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects human Tau. Other species not determined.
    Sensitivity
    100 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Sample Preparation

    Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all tau ELISA Kits
    tau (tau Protein)
    Alternative Name
    tau (tau Products)
    Synonyms
    DDPAC ELISA Kit, FTDP-17 ELISA Kit, MAPTL ELISA Kit, MSTD ELISA Kit, MTBT1 ELISA Kit, MTBT2 ELISA Kit, PPND ELISA Kit, TAU ELISA Kit, tau ELISA Kit, PHF-tau ELISA Kit, microtubule associated protein tau ELISA Kit, MAPT ELISA Kit
    Background
    Gene Names: MAPT MAPTL MTBT1 TAU
    Protein names: Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
    Gene ID
    4137
    UniProt
    P10636
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