alpha 2 Antiplasmin ELISA Kit

Catalog No. ABIN2703454

Product Details alpha 2 Antiplasmin ELISA Kit

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.12-30 ng/mL

Minimum Detection Limit: 0.12 ng/mL

Application: ELISA

Purpose: Human SERPINF2 (Alpha-2-Antiplasmin) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes human SERPINF2. Other species not yet determined.

Sensitivity: 0.12 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples200 - 2,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: Serpin F2 (SERPINF2 Products)

Synonyms: A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, serpin ELISA Kit, AI747498 ELISA Kit, Pli ELISA Kit, Serpimf2 ELISA Kit, serpinf2 ELISA Kit, serpinf2l ELISA Kit, wu:fd57d08 ELISA Kit, Dmrs91 ELISA Kit, Pedf ELISA Kit, serpin family F member 2 ELISA Kit, serpin family F member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 2 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a ELISA Kit, SERPINF2 ELISA Kit, Serpinf2 ELISA Kit, SERPINF1 ELISA Kit, serpinf2a ELISA Kit, Serpinf1 ELISA Kit

Background: Gene Names: SERPINF2 AAP PLI
Protein names: Alpha-2-antiplasmin (Alpha-2-AP) (Alpha-2-plasmin inhibitor) (Alpha-2-PI) (Serpin F2)

Gene ID: 5345

UniProt: P08697

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones