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SCARB2 ELISA Kit
SCARB2
Reactivity: Human
Colorimetric
Sandwich ELISA
10-2500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details SCARB2 ELISA Kit
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Target
See all SCARB2 ELISA Kits
SCARB2
(Scavenger Receptor Class B, Member 2 (SCARB2))
Reactivity
All reactivities for SCARB2 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
10-2500 pg/mL
Minimum Detection Limit
10 pg/mL
Application
ELISA
Purpose
Human LIMPII ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity
10 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SCARB2
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Target
See all SCARB2 ELISA Kits
SCARB2
(Scavenger Receptor Class B, Member 2 (SCARB2))
Alternative Name
LIMP-II (SCARB2 Products )
Synonyms
cd36l2 ELISA Kit, fi13c07 ELISA Kit, chunp6914 ELISA Kit, wu:fi13c07 ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, AMRF ELISA Kit, CD36L2 ELISA Kit, EPM4 ELISA Kit, HLGP85 ELISA Kit, LGP85 ELISA Kit, LIMP-2 ELISA Kit, LIMPII ELISA Kit, SR-BII ELISA Kit, 9330185J12Rik ELISA Kit, Cd36l2 ELISA Kit, MLGP85 ELISA Kit, LimpII ELISA Kit, scavenger receptor class B, member 2a ELISA Kit, scavenger receptor class B member 2 ELISA Kit, scavenger receptor class B, member 2 ELISA Kit, scarb2a ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, Scarb2 ELISA Kit
Background
Gene Names: SCARB2 CD36L2 LIMP2 LIMPII Protein names: Lysosome membrane protein 2 (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (CD36 antigen-like 2) (Lysosome membrane protein II) (LIMP II) (Scavenger receptor class B member 2) (CD antigen CD36)
Gene ID
950
UniProt
Q14108
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