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LDLR ELISA Kit
LDLR
Reactivity: Human
Colorimetric
Sandwich ELISA
8-2.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details LDLR ELISA Kit
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Target
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LDLR
(Low Density Lipoprotein Receptor (LDLR))
Reactivity
All reactivities for LDLR elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
8-2.000 pg/mL
Minimum Detection Limit
8 pg/mL
Application
ELISA
Purpose
Human LDL R ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects human LDLR. Other species not determined yet.
Sensitivity
8 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples40-400 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for LDLR
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Target
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LDLR
(Low Density Lipoprotein Receptor (LDLR))
Alternative Name
LDL R (LDLR Products )
Synonyms
FH ELISA Kit, FHC ELISA Kit, LDLCQ2 ELISA Kit, Hlb301 ELISA Kit, LDLRA ELISA Kit, LDLA ELISA Kit, LDL receptor-2 ELISA Kit, fhc ELISA Kit, ldlcq2 ELISA Kit, ldlr ELISA Kit, ldlr2-a ELISA Kit, LDL receptor 1 ELISA Kit, ldlr-a ELISA Kit, ldlr-b ELISA Kit, low density lipoprotein receptor ELISA Kit, low density lipoprotein receptor a ELISA Kit, low density lipoprotein receptor S homeolog ELISA Kit, low density lipoprotein receptor L homeolog ELISA Kit, LDLR ELISA Kit, Ldlr ELISA Kit, ldlra ELISA Kit, ldlr.S ELISA Kit, ldlr.L ELISA Kit
Background
Gene Names: LDLR Protein names: Low-density lipoprotein receptor (LDL receptor)
Gene ID
3949
UniProt
P01130
Pathways
Hepatitis C , Lipid Metabolism
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