CSF3R ELISA Kit

Catalog No. ABIN2703035

Product Details CSF3R ELISA Kit

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.1-25 ng/mL

Minimum Detection Limit: 0.1 ng/mL

Application: ELISA

Purpose: Human GCSF R (CD114) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes human G-CSF R/CD114.

Sensitivity: 0.1 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CSF3R

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Alternative Name: G-CSF R (CSF3R Products)

Synonyms: CD114 ELISA Kit, GCSFR ELISA Kit, Cd114 ELISA Kit, Csfgr ELISA Kit, G-CSF-R ELISA Kit, G-CSFR ELISA Kit, CSF3R ELISA Kit, gcsfr ELISA Kit, cd114 ELISA Kit, colony stimulating factor 3 receptor ELISA Kit, colony stimulating factor 3 receptor (granulocyte) ELISA Kit, colony stimulating factor 3 receptor L homeolog ELISA Kit, granulocyte colony-stimulating factor receptor ELISA Kit, CSF3R ELISA Kit, Csf3r ELISA Kit, csf3r ELISA Kit, csf3r.L ELISA Kit, LOC100433338 ELISA Kit

Background: Gene Names: CSF3R GCSFR
Protein names: Granulocyte colony-stimulating factor receptor (G-CSF receptor) (G-CSF-R) (CD antigen CD114)

Gene ID: 1441

UniProt: Q99062