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Contactin 1 ELISA Kit

CNTN1 Reactivity: Human Colorimetric Sandwich ELISA 0.06-15 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702920
  • Target See all Contactin 1 (CNTN1) ELISA Kits
    Contactin 1 (CNTN1)
    Reactivity
    • 6
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.06-15 ng/mL
    Minimum Detection Limit
    0.06 ng/mL
    Application
    ELISA
    Purpose
    Human Contactin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human Contactin-1 (CNTN1)
    Sensitivity
    0.06 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples20 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Contactin 1 (CNTN1) ELISA Kits
    Contactin 1 (CNTN1)
    Alternative Name
    CNTN1 (CNTN1 Products)
    Synonyms
    F3 ELISA Kit, GP135 ELISA Kit, AW495098 ELISA Kit, CNTN ELISA Kit, F3cam ELISA Kit, F3/F11 ELISA Kit, cntn-A ELISA Kit, F11 ELISA Kit, CNTN1 ELISA Kit, cntn1 ELISA Kit, contactin 1 ELISA Kit, contactin 1 L homeolog ELISA Kit, contactin 1a ELISA Kit, CNTN1 ELISA Kit, Cntn1 ELISA Kit, cntn1.L ELISA Kit, cntn1a ELISA Kit
    Background
    Gene Names: CNTN1
    Protein names: Contactin-1 (Glycoprotein gp135) (Neural cell surface protein F3)
    Gene ID
    1272
    UniProt
    Q12860
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