Cathepsin L ELISA Kit

Catalog No. ABIN2702883

Product Details Cathepsin L ELISA Kit

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 24-6.000 pg/mL

Minimum Detection Limit: 24 pg/mL

Application: ELISA

Purpose: Human Cathepsin L ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4, ADAM-9, ANG-2, APRIL, BMP-2, BMP-9, C5a, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGF R, IFN-alpha/beta R2, IGF-II, IGF-IIR, IL-1 R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRP-5, Serpin A4, sFRP-3, Thrombomodulin, TLR2, TRAIL R1, Transferrin, WIF-1.

Sensitivity: 24 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L (CTSL1 Products)

Synonyms: CATL ELISA Kit, CTSL1 ELISA Kit, MEP ELISA Kit, 1190035F06Rik ELISA Kit, Ctsl1 ELISA Kit, fs ELISA Kit, nkt ELISA Kit, CATHL ELISA Kit, CatL ELISA Kit, Ctsl ELISA Kit, CTSL ELISA Kit, mep ELISA Kit, catl ELISA Kit, ctsl ELISA Kit, cpl-1 ELISA Kit, MGC80629 ELISA Kit, MGC107932 ELISA Kit, LOC663234 ELISA Kit, 26-29-p ELISA Kit, Cp1 ELISA Kit, si:dkey-121a11.6 ELISA Kit, CTSV ELISA Kit, catL ELISA Kit, cb15 ELISA Kit, ctsl1b ELISA Kit, hgg1 ELISA Kit, wu:fb30g09 ELISA Kit, zgc:92089 ELISA Kit, cathepsin L ELISA Kit, cathepsin V ELISA Kit, cathepsin L L homeolog ELISA Kit, cathepsin L1 ELISA Kit, Cathepsin L1 ELISA Kit, Cathepsin L ELISA Kit, testin gene ELISA Kit, cathepsin 12 ELISA Kit, cathepsin Lb ELISA Kit, cathepsin L.1 ELISA Kit, CTSL ELISA Kit, Ctsl ELISA Kit, CTSV ELISA Kit, ctsl.L ELISA Kit, ctsl ELISA Kit, LOC100008830 ELISA Kit, catl1 ELISA Kit, 26-29-p ELISA Kit, catl ELISA Kit, Tsp_11596 ELISA Kit, Testin ELISA Kit, cts12 ELISA Kit, LOC102156614 ELISA Kit, ctslb ELISA Kit, ctsl.1 ELISA Kit

Background: Gene Names: CTSL CTSL1
Protein names: Cathepsin L1 (EC 3.4.22.15) (Cathepsin L) (Major excreted protein) (MEP) [Cleaved into: Cathepsin L1 heavy chain, Cathepsin L1 light chain]

Gene ID: 1514

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades